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Literature summary for 7.1.1.3 extracted from

  • Minghetti, K.C.; Goswitz, V.C.; Gabriel, N.E.; Hill, J.J.; Barassi, C.A.; Georgiou, C.D.; Chan, S.I.; Gennis, R.B.
    Modified, large-scale purification of the cytochrome o complex (bo-type oxidase) of Escherichia coli yields a two heme/one copper terminal oxidase with high specific activity (1992), Biochemistry, 31, 6917-6924.
    View publication on PubMed
Search for more literature for 7.1.1.3

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Escherichia coli 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ the pure four-subunit enzyme contains one equivalent of copper (7.5 ng/mg) Escherichia coli
Fe the pure four-subunit enzyme contains two equivalents of iron (19.5 ng/mg) Escherichia coli
additional information the enzyme contains no zinc. The equivalent of CuA of the aa3-type cytochrome c oxidases is absent in this quinol oxidase Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 1 * 58000 + 1 * 33000 + 1 * 22000 + 1 * 17000, SDS-PAGE Escherichia coli
22000
-
 1 * 58000 + 1 * 33000 + 1 * 22000 + 1 * 17000, SDS-PAGE Escherichia coli
33000
-
 1 * 58000 + 1 * 33000 + 1 * 22000 + 1 * 17000, SDS-PAGE Escherichia coli
58000
-
 1 * 58000 + 1 * 33000 + 1 * 22000 + 1 * 17000, SDS-PAGE Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli RG145
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
960
-
 at 37°C, pH not specified in the publication Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not interact with cytochrome c Escherichia coli ?
-
 ?
additional information the enzyme does not interact with cytochrome c Escherichia coli RG145 ?
-
 ?
ubiquinol-1 + O2 + H+/in
-
 Escherichia coli ubiquinone-1 + H2O + H+/out
-
 ?
ubiquinol-1 + O2 + H+/in
-
 Escherichia coli RG145 ubiquinone-1 + H2O + H+/out
-
 ?

Subunits

Subunits Comment Organism
heterotetramer 1 * 58000 + 1 * 33000 + 1 * 22000 + 1 * 17000, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
bo-type ubiquinol oxidase
-
 Escherichia coli
cytochrome o complex
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
heme the enzyme possesses two hemes (18.1 ng/mg) Escherichia coli