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Literature summary for 7.1.1.2 extracted from
- Apoptosis-inducing factor (AIF) and its family member protein, AMID, are rotenone-sensitive NADH ubiquinone oxidoreductases (NDH-2) (2015), J. Biol. Chem., 290, 20815-20826 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-n-heptyl-4-hydroxyquinoline-N-oxide | - |
Homo sapiens |  |
| rotenone | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
| mitochondrion | - |
Homo sapiens | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | O95831 | - |
- |
| Homo sapiens | Q9BRQ8 | - |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.77 | - |
pH 7.0, 30°C, N-terminally tagged protein | Homo sapiens |
| 7.52 | - |
pH 7.0, 30°C, N-terminally tagged protein | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + oxidized coenzyme Q1 + 5 H+[side1] | - |
Homo sapiens | NAD+ + reduced coenzyme Q1 + 4 H+[side2] | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AIF-homologous mitochondrion-associated inducer of death | - |
Homo sapiens |
| AIFM1 | - |
Homo sapiens |
| AIFM2 | - |
Homo sapiens |
| AmiD | - |
Homo sapiens |
| apoptosis-inducing factor 1 | - |
Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Homo sapiens | |
| additional information | no cofactor: deamino-NADH | Homo sapiens | |
| NADH | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00075 | - |
2-n-heptyl-4-hydroxyquinoline-N-oxide | pH 7.0, 30°C, N-terminally tagged protein | Homo sapiens | |
| 0.00104 | - |
2-n-heptyl-4-hydroxyquinoline-N-oxide | pH 7.0, 30°C, N-terminally tagged protein | Homo sapiens | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | after reconstituting isolated AIFM1 into bacterial or mitochondrial membranes, N-terminally tagged AIFM1 displays substantial NADH:O2 activity and supports NADH-linked proton pumping activities in the host membranes. Overexpressing N-terminally tagged AIFM1 and AIF-homologous mitochondrion-associated inducer of death AMID enhances the growth of a double knock-out Escherichia coli strain lacking complex I and NDH-2. C-terminally tagged AIFM1 and NADH-binding site mutants of N-terminally tagged AIFM1 and AMID fail to show both NADH:O2 activity and the growth-enhancing effect | Homo sapiens |
| physiological function | after reconstituting isolated AIFM2 into bacterial or mitochondrial membranes, N-terminally tagged AIFM2 displays substantial NADH:O2 activity and supports NADH-linked proton pumping activities in the host membranes. Overexpressing N-terminally tagged AIFM1 and AIFM2 enhances the growth of a double knock-out Escherichia coli strain lacking complex I and NDH-2. NADH-binding site mutants of N-terminally tagged AIFM1 and AIFM2 fail to show both NADH:O2 activity and the growth-enhancing effect | Homo sapiens |
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