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Literature summary for 7.1.1.2 extracted from

  • Venkatakrishnan, P.; Lencina, A.M.; Schurig-Briccio, L.A.; Gennis, R.B.
    Alternate pathways for NADH oxidation in Thermus thermophilus using type 2 NADH dehydrogenases (2013), Biol. Chem., 394, 667-676 .
    View publication on PubMed
Search for more literature for 7.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Thermus thermophilus and in Eschrichia coli Thermus thermophilus

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: rotenone; not inhibitory: rotenone Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 menadione pH 7.5, 60°C Thermus thermophilus
0.024
-
 NADH pH 7.5, 60°C Thermus thermophilus
0.032
-
 menadione pH 7.5, 60°C Thermus thermophilus
0.05
-
 NADH pH 7.5, 60°C Thermus thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Thermus thermophilus 16020
-

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q70DV6
-
-
Thermus thermophilus Q72HK3
-
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q70DV6
-
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 Q72HK3
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.17
-
 60°C, pH 7.5, recombinant protein expressed in Escherichia coli, presence of FAD Thermus thermophilus
1.23
-
 60°C, pH 7.5, recombinant protein expressed in Escherichia coli, presence of FAD Thermus thermophilus
20
-
 60°C, pH 7.5, recombinant protein expressed in Thermus thermopilus, presence of FAD Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + decylubiquinone
-
 Thermus thermophilus NAD+ + decylubiquinol
-
 ?
NADH + H+ + decylubiquinone
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 NAD+ + decylubiquinol
-
 ?
NADH + H+ + duroquinone
-
 Thermus thermophilus NAD+ + duroquinol
-
 ?
NADH + H+ + duroquinone
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 NAD+ + duroquinol
-
 ?
NADH + H+ + menadione
-
 Thermus thermophilus NAD+ + menadiol
-
 ?
NADH + H+ + menadione
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 NAD+ + menadiol
-
 ?
NADH + H+ + ubiquinone-1
-
 Thermus thermophilus NAD+ + ubiquinol-1
-
 ?
NADH + H+ + ubiquinone-1
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 NAD+ + ubiquinol-1
-
 ?
NADH + H+ + vitamin K2
-
 Thermus thermophilus NAD+ + reduced vitamin K2
-
 ?
NADH + H+ + vitamin K2
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 NAD+ + reduced vitamin K2
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, calculated Thermus thermophilus
? x * 48000, SDS-PAGE and calculated Thermus thermophilus

Synonyms

Synonyms Comment Organism
NDH-2A
-
 Thermus thermophilus
NrcN
-
 Thermus thermophilus
TT_C1484
-
 Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
 NADH pH 7.5, 60°C Thermus thermophilus
1.1
-
 NADH pH 7.5, 60°C Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
FAD FAD content of homologously produced NrcN is 1 mol of FAD/mol protein. FAD is not covalently bound Thermus thermophilus
FAD UV-Vis spectrum shows a shoulder at 465 nm. FAD is not covalently bound Thermus thermophilus

General Information

General Information Comment Organism
physiological function protein NrcN functions independently of other components of the putative Nrc complex Thermus thermophilus