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Literature summary for 7.1.1.2 extracted from

  • Virzintiene, E.; Trane, M.; Haegerhaell, C.
    Revised transmembrane orientation of the NADH:quinone oxidoreductase subunit NuoA (2011), FEBS Lett., 585, 3277-3283.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene nuoA, encoding a membrane-spanning subunit of complex I, expression in Escherichia coli strain Rosetta Gami and Jm109,expression as full-length and truncated NuoA C-terminally fused to His-tagged cytochrome c domain Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane subunit NuoA topology modeling, overview Escherichia coli 16020
-

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
gene nuoA encoding a membrane-spanning subunit of complex I
-

Purification (Commentary)

Purification (Comment) Organism
recombinant membrane-spanning subunit NuoA of complex I from Escherichia coli strain JM109 as full-length and truncated NuoA C-terminally fused to His-tagged cytochrome c domain by solubilization, ultracentrifugation, and affinity chromatography Escherichia coli

Synonyms

Synonyms Comment Organism
complex I
-
Escherichia coli
NADH:quinone oxidoreductase
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Escherichia coli

General Information

General Information Comment Organism
additional information NuoA is a small membrane spanning subunit of respiratory chain NADH:quinone oxidoreductase (complex I). Unlike the other complex I core protein subunits, the NuoA protein has no known homologue in other enzyme systems Escherichia coli