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Literature summary for 7.1.1.2 extracted from

  • Moparthi, V.K.; Kumar, B.; Mathiesen, C.; Haegerhaell, C.
    Homologous protein subunits from Escherichia coli NADH:quinone oxidoreductase can functionally replace MrpA and MrpD in Bacillus subtilis (2011), Biochim. Biophys. Acta, 1807, 427-436.
    View publication on PubMed
Search for more literature for 7.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression of recombinant enzyme subunits in Escherichia coli, quantitative expression analysis Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information construction of mrpA or mrpD deletion mutant strains, the deletion strains can be complemented in trans by their respective Mrp protein, but expression of MrpA in the Bacillus subtilis DELTAmrpD strain and vice versa does not improve growth at pH 7.4, mutant strains growth phenotypes, overview Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + ubiquinone + 6 H+[side 1] Bacillus subtilis
-
 NAD+ + ubiquinol + 7 H+[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
 genes mrpA or mrpD
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + ubiquinone + 6 H+[side 1]
-
 Bacillus subtilis NAD+ + ubiquinol + 7 H+[side 2]
-
 ?

Synonyms

Synonyms Comment Organism
complex I
-
 Bacillus subtilis
NADH:ubiquinone oxidoreductase
-
 Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Bacillus subtilis

General Information

General Information Comment Organism
evolution the complex I subunits NuoL, NuoM and NuoN are homologous to two proteins, MrpA and MrpD, from one particular class of Na+/H+ antiporters. In many bacteria MrpA and MrpD are encoded by an operon comprising 6-7 conserved genes Bacillus subtilis
malfunction deletion of either mrpA or mrpD from the Bacillus subtilis chromosome resulted in a Na+ and pH sensitive growth phenotype. NuoL can rescue Bacillus subtilis DELTAmrpA, but improved the growth of Bacillus subtilis DELTAmrpD only slightly. NuoN can restore the wild type properties of Bacillus subtilis DELTAmrpD, but has no effect on the ?mrpA strain. Expression of NuoM does not result in any growth improvement under these conditions. This reveals that the complex I subunits NuoL, NuoM and NuoN also demonstrate functional specializations Bacillus subtilis
physiological function MrpA transports Na+ whereas MrpD transports H+ in opposite directions, resulting in antiporter activity, besides one Na+ channel, NuoL, and two H+ channels, NuoM and NuoN, are present. Bacillus subtilis