Literature summary for 7.1.1.2 extracted from

Mogi, T.; Murase, Y.; Mori, M.; Shiomi, K.; Omura, S.; Paranagama, M.P.; Kita, K.
Polymyxin B identified as an inhibitor of alternative NADH dehydrogenase and malate: quinone oxidoreductase from the Gram-positive bacterium Mycobacterium smegmatis (2009), J. Biochem., 146, 491-499.

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Inhibitors

Inhibitors	Comment	Organism	Structure
nanaomycin A	-	Mycolicibacterium smegmatis
Polymyxin B	in the presence of polymyxin B, enzyme kinetics changes from the MichaelisMenten type to substrate inhibition kinetics with the substrate inhibition	Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.015	-	ubiquinone-1	pH 7.4, 25°C	Mycolicibacterium smegmatis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Mycolicibacterium smegmatis	16020	-

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADH + H+ + ubiquinone-1	-	Mycolicibacterium smegmatis	NAD+ + ubiquinol-1	-	?

Synonyms

Synonyms	Comment	Organism
NDH2	-	Mycolicibacterium smegmatis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Mycolicibacterium smegmatis

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
additional information	-	IC50 for polymyxin B is 0.0014 mg/ml, IC50 for nanaomycin A is 0.031 mg/ml	Mycolicibacterium smegmatis	additional information

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Release 2023.1 (January 2023)