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Literature summary for 7.1.1.2 extracted from
- Superoxide radical formation by pure complex I (NADH:ubiquinone oxidoreductase) from Yarrowia lipolytica (2005), J. Biol. Chem., 280, 30129-30135.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| R141M | reduced activity compared to wild type enzyme | Yarrowia lipolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-decyl-4-quinazolinyl amine | 0.00022 mM | Yarrowia lipolytica |  |
| antimycin A | 0.0009 mM | Yarrowia lipolytica | |
| diphenyleineiodonium | does not inhibit superoxide generation | Yarrowia lipolytica | |
| rotenone | does not inhibit superoxide generation | Yarrowia lipolytica | |
| Sodium azide | 10 mM | Yarrowia lipolytica | |
| Stigmatellin | 0.001 mM | Yarrowia lipolytica | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Yarrowia lipolytica | 5739 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Yarrowia lipolytica | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.3 | - |
mutant enzyme R141M, NADH-dependent activity of complex 1 containing proteoliposomes using n-decylubiquinone as substrate | Yarrowia lipolytica |
| 5.5 | - |
wild type enzyme, NADH-dependent activity of complex 1 containing proteoliposomes using n-decylubiquinone as substrate | Yarrowia lipolytica |
| 16.1 | - |
mutant enzyme R141M, NADH-dependent activity of complex 1 containing proteoliposomes using hexaammineruthenium-(III)-chloride as substrate | Yarrowia lipolytica |
| 23 | - |
wild type enzyme, NADH-dependent activity of complex 1 containing proteoliposomes using hexaammineruthenium-(III)-chloride as substrate | Yarrowia lipolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + hexaammineruthenium-(III)-chloride | - |
Yarrowia lipolytica | NAD+ + ? | - |
? | |
| NADH + n-decylubiquinone + H+ | - |
Yarrowia lipolytica | NAD+ + n-decylubiquinol | - |
? | |
| NADH + O2 | - |
Yarrowia lipolytica | NAD+ + superoxide radical | - |
? | |
| NADH + ubiquinone | - |
Yarrowia lipolytica | NAD+ + ubiquinol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| complex I | - |
Yarrowia lipolytica |
| NADH:ubiquinone oxidoreductase | - |
Yarrowia lipolytica |
| type I NADH dehydrogenase | - |
Yarrowia lipolytica |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | - |
Yarrowia lipolytica | |
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Release 2023.1 (January 2023)
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