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Literature summary for 7.1.1.2 extracted from
- Agrobacterium tumefaciens type II NADH dehydrogenase. Characterization and interactions with bacterial and thylakoid membranes (2006), FEBS J., 273, 3625-3637.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Agrobacterium tumefaciens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diphenylene iodonium | 75% inhibition, IC50: 0.013 mM | Agrobacterium tumefaciens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.005 | - |
NADH | in the presence of 1 mM ferricyanide | Agrobacterium tumefaciens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
1 * 46000, SDS-PAGE | Agrobacterium tumefaciens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agrobacterium tumefaciens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-affinity chromatography | Agrobacterium tumefaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 ferricyanide + NADH | - |
Agrobacterium tumefaciens | 2 ferrocyanide + NAD+ + H+ | - |
? | |
| decyl-ubiquinone + NADH | - |
Agrobacterium tumefaciens | decyl-ubiquinol + NAD+ | - |
? | |
| NADH + plastoquinone | - |
Agrobacterium tumefaciens | NAD+ + plastoquinol | - |
? | |
| NADH + ubiquinone | - |
Agrobacterium tumefaciens | NAD+ + ubiquinol | - |
? | |
| NADPH + H+ + ubiquinone | the enzyme has a lower affinity to NADPH than to NADH | Agrobacterium tumefaciens | NADP+ + ubiquinol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 46000, SDS-PAGE | Agrobacterium tumefaciens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NDH-2 | - |
Agrobacterium tumefaciens |
| type II NADH dehydrogenase | - |
Agrobacterium tumefaciens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Agrobacterium tumefaciens | |
| NADH | - |
Agrobacterium tumefaciens | |
| NADPH | much higher affinity for NADH than for NADPH | Agrobacterium tumefaciens | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.013 | - |
75% inhibition, IC50: 0.013 mM | Agrobacterium tumefaciens | diphenylene iodonium | |
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Release 2023.1 (January 2023)
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