Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 7.1.1.2 extracted from

  • Scheide, D.; Huber, R.; Friedrich, T.
    The proton-pumping NADH:ubiquinone oxidoreductase (complex I) of Aquifex aeolicus (2002), FEBS Lett., 512, 80-84.
    View publication on PubMed
Search for more literature for 7.1.1.2

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 ubiquinone-2
-
 Aquifex aeolicus
0.012
-
 NADH
-
 Aquifex aeolicus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Aquifex aeolicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
550000
-
 sucrose density gradient centrifugation Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2
-
 NADH:duroquinone reductase activity Aquifex aeolicus
1.3
-
 50°C, 0.02 mM ubiquinone-2 as substrate Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NADH
-
 Aquifex aeolicus 2 ferrocyanide + NAD+ + H+
-
 ?
NADH + duroquinone
-
 Aquifex aeolicus NAD+ + duroquinol
-
 ?
NADH + ubiquinone-2
-
 Aquifex aeolicus NAD+ + ubiquinol-2
-
 ?

Synonyms

Synonyms Comment Organism
complex I
-
 Aquifex aeolicus
proton-pumping NADH:ubiquinone oxidoreductase
-
 Aquifex aeolicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Aquifex aeolicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Aquifex aeolicus

pH Range

pH Minimum pH Maximum Comment Organism
8 10.3 pH 8.0: about 60% of maximal activity, pH 10.3: about 30% of maximal activity Aquifex aeolicus

Cofactor

Cofactor Comment Organism Structure
NADH no activity with NADPH Aquifex aeolicus