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Literature summary for 7.1.1.2 extracted from

  • Kerscher, S.; Grgic, L.; Garofano, A.; Brandt, U.
    Application of the yeast Yarrowia lipolytica as a model to analyse human pathogenic mutations in mitochondrial complex I (NADH:ubiquinone oxidoreductase) (2004), Biochim. Biophys. Acta, 1659, 197-205.
    View publication on PubMed
Search for more literature for 7.1.1.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Yarrowia lipolytica

Protein Variants

Protein Variants Comment Organism
E107A mutation in the 49000 Da subunit, no effect on complex I contant, mutant enzyme displays no deamino-nicotinamide-adeninedinucleotide:N-decylubiquinone activity Yarrowia lipolytica
F87L mutation in the 49000 Da subunit, no effect on complex I content, deamino-nicotinamide-adeninedinucleotide:N-decylubiquinone activity is reduced to aboput 60% of the parental strain value. The KM-value for n-decylubiquinone and the I50 value for rotenone are noemal Yarrowia lipolytica
P232Q mutation in thge 49000 Da subunit. Complex I assembly is severly impaired in this mutant. Deamino-nicotinamide-adeninedinucleotide:N-decylubiquinone activity is less than 20% of the normal value Yarrowia lipolytica
R199W mutation in the 30000 Da subunit, no significant alterations in complex I content or activity can be abserved in isolated mitochondrial membranes Yarrowia lipolytica
R231E mutation in the 49000 Da subunit of the complex, mutation has no effect on complex I content. Its deamino-nicotinamide-adeninedinucleotide:N-decylubiquinone activity is slightly reduced and its Km-value is somewhat elevated Yarrowia lipolytica
R231Q mutation in the 49000 Da subunit of the complex, no difference to wild-type enzyme in activity and stability Yarrowia lipolytica
S416A mutation in the 49000 Da subunit of the complex, mutation has no effect on complex I content. Its deamino-nicotinamide-adeninedinucleotide:N-decylubiquinone activity is slightly reduced. KM-value and I50 value for rotenone are both significantly higher than in the parental strain Yarrowia lipolytica
S416P mutation in the 49000 Da subunit of the complex, no difference to wild-type enzyme in activity and stability Yarrowia lipolytica
T157I mutation in the 30000 Da subunit, no significant alterations in complex I content or activity can be abserved in isolated mitochondrial membranes Yarrowia lipolytica

Inhibitors

Inhibitors Comment Organism Structure
2-decyl-4-quinazolinyl amine
-
 Yarrowia lipolytica
rotenone
-
 Yarrowia lipolytica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
 n-decylubiquinone mutant P232G (49000 Da subunit) Yarrowia lipolytica
0.012
-
 n-decylubiquinone mutant T157I (30000 Da subunit) Yarrowia lipolytica
0.014
-
 n-decylubiquinone mutant R211W (30000 Da subunit) Yarrowia lipolytica
0.016
-
 n-decylubiquinone mutant D228Q (49000 Da subunit) Yarrowia lipolytica
0.017
-
 n-decylubiquinone mutant S416P (49000 Da subunit) Yarrowia lipolytica
0.021
-
 n-decylubiquinone mutant F87L (49000 Da subunit) Yarrowia lipolytica
0.029
-
 n-decylubiquinone mutant R231E (49000 Da subunit) Yarrowia lipolytica
0.039
-
 n-decylubiquinone mutant S416A (49000 Da subunit) Yarrowia lipolytica

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Yarrowia lipolytica
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deamino-nicotinamide-adeninedinucleotide + n-decylubiquinone
-
 Yarrowia lipolytica ? + n-decylubiquinol
-
 ?

Synonyms

Synonyms Comment Organism
complex I
-
 Yarrowia lipolytica
NADH:ubiquinone oxidoreductase
-
 Yarrowia lipolytica