Any feedback?
Literature summary for 7.1.1.2 extracted from
- Expression and characterization of the 66-kilodalton (NQO3) ison-sulfur subunit of the proton-translocating NADH-quinone oxidoreducrase of Paracoccus denitrificans (1995), J. Biol. Chem., 270, 18264-18270.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| NQO3 subunit overexpressed in NQO3 | Paracoccus denitrificans |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | the enzyme contains at least 5 EPR-visible iron-sulfur clusters. The NQO3 subunits contains at least two distinct iron-sulfur clusters: 1 [2Fe-2S] cluster with axial EPR signals and a [4Fe-4S] cluster with rhombic symmetry. The midpoint redox potentials of [2Fe-2S] and [4Fe-4S] clusters at pH 8.6 is -472 mV and -391 mV, respectively | Paracoccus denitrificans |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + ubiquinone | Paracoccus denitrificans | proton-translocating enzyme | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus denitrificans | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| the tetranuclear cluster in the isolated NQO3 subunit is sensitive towards oxidants and converts into [3Fe-4S] form | Paracoccus denitrificans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant NQO3 subunit | Paracoccus denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + ubiquinone | proton-translocating enzyme | Paracoccus denitrificans | NAD+ + ubiquinol | - |
? | |
| NADH + ubiquinone | proton-translocating enzyme | Paracoccus denitrificans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | proton-translocating NADH-quinone oxidoreductase is composed of at least 14 dissimilar subunits, designated NQO1-14. NQO1, NQO2, NQO3, NQO9, and probably NQO6 subunits are cofactor binding subunits | Paracoccus denitrificans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADH-quinone oxidoreductase | - |
Paracoccus denitrificans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FMN | enzyme contains one noncovalently bound FMN | Paracoccus denitrificans | |
| NADH | - |
Paracoccus denitrificans | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
