Any feedback?
Literature summary for 7.1.1.2 extracted from
- Kinetic characterization of the rotenone-insensitive internal NADH:ubiquinone oxidoreductase of mitochondria from Saccharomyces cerevisiae (2001), Arch. Biochem. Biophys., 389, 7-14.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | - |
Saccharomyces cerevisiae |  |
| AMP | dead-end inhibitor, linear competitive inhibitor of NADH, linear uncompetitive inhibitor of oxidized 2,6-dichlorophenol indophenol | Saccharomyces cerevisiae | |
| flavone | dead-end inhibitor; partial inhibitor displaying a hyperbolic uncompetitive inhibition with respect to oxidized 2,6-dichlorophenol indophenol | Saccharomyces cerevisiae | |
| reduced 2,6-dichlorophenolindophenol | - |
Saccharomyces cerevisiae | |
| rotenone | rotenone-insensitive enzyme | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0062 | - |
oxidized 2,6-dichlorophenolindophenol | - |
Saccharomyces cerevisiae | |
| 0.0094 | - |
NADH | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
x * 53000, SDS-PAGE | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + ubiquinone | Saccharomyces cerevisiae | the enzyme catalyzes the transfer of electrons without translocation of protons across the membrane | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
rotenone-insensitive enzyme | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| NADH + ubiquinone + 6 H+[side 1] = NAD+ + ubiquinol + 7 H+[side 2] | ping-pong mechanism | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| NADH + oxidized 2,6-dichlorophenolindophenol | - |
Saccharomyces cerevisiae | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
| NADH + oxidized 2,6-dichlorophenolindophenol + H+ | 2,6-dichlorophenol indophenol as electron acceptor | Saccharomyces cerevisiae | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
| NADH + ubiquinone | - |
Saccharomyces cerevisiae | NAD+ + ubiquinol | - |
? | |
| NADH + ubiquinone | the enzyme catalyzes the transfer of electrons without translocation of protons across the membrane | Saccharomyces cerevisiae | ? | - |
? | |
| NADH + ubiquinone-6 | - |
Saccharomyces cerevisiae | NAD+ + ubiquinol-6 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000, SDS-PAGE | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | enzyme contains FAD | Saccharomyces cerevisiae | |
| NADH | - |
Saccharomyces cerevisiae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
2,6-dichlorophenolindophenol | - |
Saccharomyces cerevisiae | |
| 5.5 | - |
AMP | versus NADH | Saccharomyces cerevisiae | |
| 11.5 | - |
AMP | versus oxidized 2,6-dichlorophenolindophenol | Saccharomyces cerevisiae | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
