Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM83 cells | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N,N'-dicyclohexylcarbodiimide | modification of the enzyme with N,N'-dicyclohexylcarbodiimide leads to inhibition of the rate of release of NADP+ and NADPH from the enzyme, but has a much smaller effect on the binding and release of NAD+, NADH and their analogues and on the interconversion of the ternary complexes of the enzyme with its substrates | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
1 * 54000 + 1 * 49000, SDS-PAGE | Escherichia coli |
54000 | - |
1 * 54000 + 1 * 49000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ + H+[side 1] | Escherichia coli | - |
NADP+ + NADH + H+[side 2] | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phenyl-Sepharose column chromatography and DEAE-Trisacryl M column chromatography | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.11 | - |
with oxidized acetyl pyridine adenine dinucleotide and deamino-NADPH as substrates, at pH 6.0 and 25°C | Escherichia coli |
0.47 | - |
with oxidized acetyl pyridine adenine dinucleotide and 1-N6-etheno-NADPH as substrates, at pH 6.0 and 25°C | Escherichia coli |
1.46 | - |
with oxidized acetyl pyridine adenine dinucleotide and NADPH as substrates, at pH 6.0 and 25°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,N6-etheno-NADPH + NAD+ + H+[side 1] | - |
Escherichia coli | 1,N6-etheno-NADP+ + NADH + H+[side 2] | - |
r | |
1,N6-etheno-NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1] | - |
Escherichia coli | 1,N6-etheno-NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2] | - |
r | |
deamino-NADPH + NAD+ + H+[side 1] | - |
Escherichia coli | deamino-NADP+ + NADH + H+[side 2] | - |
r | |
deamino-NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1] | - |
Escherichia coli | deamino-NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2] | - |
r | |
NADPH + NAD+ + H+[side 1] | - |
Escherichia coli | NADP+ + NADH + H+[side 2] | - |
r | |
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1] | - |
Escherichia coli | NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2] | - |
r |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 54000 + 1 * 49000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
H+-transhydrogenase | - |
Escherichia coli |
proton-translocating transhydrogenase | - |
Escherichia coli |