Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
Y146A | mutation in component dI that binds NADH. dI.Y146A more readily dissociates into monomers than wild-type dI. dI.Y146A monomers bind NADH much more weakly than dimers. dI.Y146A reconstitutes activity to dI-depleted membranes in its dimeric form but not in its monomeric form | Rhodospirillum rubrum |
Y146A | the mutant binds NADH much more weakly than the wild type enzyme | Rhodospirillum rubrum |
Y146F | mutation in component dI that binds NADH. Wild-type dI and dI.Y146F reconstituted activity to dI-depleted membranes with similar characteristics | Rhodospirillum rubrum |
Y146F | the mutant shows wild type NADH binding ability | Rhodospirillum rubrum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rhodospirillum rubrum | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40300 | - |
2 * 40300, calculated from amino acid sequence | Rhodospirillum rubrum |
84000 | - |
gel filtration | Rhodospirillum rubrum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ + H+[side 1] | Rhodospirillum rubrum | - |
NADP+ + NADH + H+[side 2] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADPH + NAD+ + H+[side 1] | - |
Rhodospirillum rubrum | NADP+ + NADH + H+[side 2] | - |
? | |
NADPH + oxidized acetyl pyridine adenine dinucleotide + H+[side 1] | - |
Rhodospirillum rubrum | NADP+ + reduced acetyl pyridine adenine dinucleotide + H+[side 2] | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40300, calculated from amino acid sequence | Rhodospirillum rubrum |
More | the protein has three components: dI binds NADH, dIII binds NADP+, and dII spans the membrane. Transhydrogenase is a dimer of two dI-dII-dIII monomers. The two catalytic sites alternate during turnover | Rhodospirillum rubrum |
Synonyms | Comment | Organism |
---|---|---|
proton-translocating transhydrogenase | - |
Rhodospirillum rubrum |
transhydrogenase | - |
Rhodospirillum rubrum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-15 | - |
the enzyme remains stable for more than 3 months at -15°C | Rhodospirillum rubrum |
4 | - |
the enzyme remains stable for more than 2 days at 4°C | Rhodospirillum rubrum |