Literature summary for 7.1.1.1 extracted from

Egorov, M.V.; Tigerstrom, A.; Pestov, N.B.; Korneenko, T.V.; Kostina, M.B.; Shakhparonov, M.I.; Rydstrom, J.
Purification of a recombinant membrane protein tagged with a calmodulin-binding domain: properties of chimeras of the Escherichia coli nicotinamide nucleotide transhydrogenase and the C-terminus of human plasma membrane Ca2+ -ATPase (2004), Protein Expr. Purif., 36, 31-39.

Search for more literature for 7.1.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
fusion protein with calmodulin-binding peptide and His-tag expressed in Escherichia coli JM109	Escherichia coli

General Stability

General Stability	Organism
fusion protein is substantially more stable than wild type enzyme upon storage at 4°C	Escherichia coli

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Escherichia coli	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
NADH + NADP+	Escherichia coli	links hydride transfer between NAD(H) and NADP(H) to the outside in translocation of protons across membrane	NAD+ + NADPH	-	r

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	fusion protein with calmodulin-binding peptide	-

Purification (Commentary)

Purification (Comment)	Organism
fusion protein using His-tag and on calmodulin-sepharose	Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
10.8	-	purified enzyme, pH 7.3	Escherichia coli

Storage Stability

Storage Stability	Organism
4°C, fusion protein is substantially more stable than wild type enzyme upon storage	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NADH + NADP+	-	Escherichia coli	NAD+ + NADPH	-	r
NADH + NADP+	links hydride transfer between NAD(H) and NADP(H) to the outside in translocation of protons across membrane	Escherichia coli	NAD+ + NADPH	-	r
thio-NADH + NADP+	-	Escherichia coli	thio-NAD+ + NADPH	-	r

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Escherichia coli

pH Range

pH Minimum	pH Maximum	Comment	Organism
5.5	8.5	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NAD(P)+	-	Escherichia coli
NAD(P)H	-	Escherichia coli

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Release 2023.1 (January 2023)