Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli MC4100TH- | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
G132A | in domain dII, no effect on wild type reverse activity | Escherichia coli |
G138A | in domain dII, 57% of wild type reverse activity | Escherichia coli |
G226A | in domain dII, 50% of wild type reverse activity | Escherichia coli |
G233A | in domain dII, 49% of wild type reverse activity | Escherichia coli |
G245A | in domain dII, no effect of wild type reverse activity | Escherichia coli |
G249A | in domain dII, 79% wild type reverse activity | Escherichia coli |
G252A | in domain dII, 2.6% of wild type reverse activity | Escherichia coli |
G252C | in domain dII, 1.9% of wild type reverse activity | Escherichia coli |
G252S | in domain dII, 2.4% of wild type reverse activity | Escherichia coli |
G252T | in domain dII, 2.3% of wild type reverse activity | Escherichia coli |
G252V | in domain dII, 2.5% of wild type reverse activity | Escherichia coli |
G95A | in domain dII, 56% of wild type reverse activity | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Escherichia coli | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + NADP+ | Escherichia coli | links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane, provides NADPH for the reduction of glutathione, reverse reaction results in outward proton translocation and creation of a proton motive force | NAD+ + NADPH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + NADP+ | - |
Escherichia coli | NAD+ + NADPH | - |
r | |
NADH + NADP+ | links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane, provides NADPH for the reduction of glutathione, reverse reaction results in outward proton translocation and creation of a proton motive force | Escherichia coli | NAD+ + NADPH | - |
r |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Escherichia coli | |
NAD(P)H | - |
Escherichia coli |