Cloned (Comment) | Organism |
---|---|
domains expressed separately in Escherichia coli | Rhodospirillum rubrum |
Crystallization (Comment) | Organism |
---|---|
(dI.Q132N)2dIII trimer | Rhodospirillum rubrum |
Protein Variants | Comment | Organism |
---|---|---|
E155W | dIII domain | Rhodospirillum rubrum |
Q132N | dI domain, no cyclic transhydrogenation activity in mixtures of domain dIII with the dI mutant, mutation has little effect on the NADH binding affinity | Rhodospirillum rubrum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rhodospirillum rubrum | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + NADP+ | Rhodospirillum rubrum | links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane | NAD+ + NADPH | - |
r | |
NADPH + oxidized 3-acetylpyridine adenine dinucleotide | Rhodospirillum rubrum | - |
NADP+ + reduced 3-acetylpyridine adenine dinucleotide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodospirillum rubrum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant proteins | Rhodospirillum rubrum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + NADP+ | - |
Rhodospirillum rubrum | NAD+ + NADPH | - |
r | |
NADH + NADP+ | links hydride transfer between NAD(H) and NADP(H) to the translocation of protons across membrane | Rhodospirillum rubrum | NAD+ + NADPH | - |
r | |
NADPH + oxidized 3-acetylpyridine adenine dinucleotide | - |
Rhodospirillum rubrum | NADP+ + reduced 3-acetylpyridine adenine dinucleotide | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | mixture of isolated domains dI and dIII forms dI2dIII trimers, formation of trimers also with Q132N mutant dI domain | Rhodospirillum rubrum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Rhodospirillum rubrum | |
NAD(P)H | - |
Rhodospirillum rubrum |