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Literature summary for 7.1.1.1 extracted from

  • Cotton, N.P.J.; Lever, T.M.; Nore, B.F.; Jones, M.R.; Jackson, J.B.
    The coupling between protonmotive force and the NAD(P)+ transhydrogenase in chromatophores from photosynthetic bacteria [published erratum appears in Eur J Biochem 1989 Oct 1;184(3):729] (1989), Eur. J. Biochem., 182, 593-603.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
light forward reaction in chromatophores is accelerated more than 20fold during illumination with photosynthetically active light Rhodobacter capsulatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
thio-NADP+ pH 6.3, in the light Rhodobacter capsulatus
0.013
-
thio-NADP+ pH 7.6, in the light Rhodobacter capsulatus
0.019
-
thio-NADP+ pH 7.6, in the dark Rhodobacter capsulatus
0.02
-
NADH pH 6.3, in the light Rhodobacter capsulatus
0.022
-
NADH pH 6.3, in the dark Rhodobacter capsulatus
0.025
-
NADH pH 7.6, in the light Rhodobacter capsulatus
0.029
-
thio-NADP+ pH 9.0, in the light Rhodobacter capsulatus
0.032
-
thio-NADP+ pH 6.3, in the dark Rhodobacter capsulatus
0.033
-
NADH pH 9.0, in the light Rhodobacter capsulatus
0.045
-
thio-NADP+ pH 9.0, in the dark Rhodobacter capsulatus
0.055
-
thio-NADP+ pH 10.0, in the light Rhodobacter capsulatus
0.064
-
NADH pH 7.6, in the dark Rhodobacter capsulatus
0.066
-
NADH pH 9.0, in the dark Rhodobacter capsulatus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required for catalytic activity Rhodobacter capsulatus
Ca2+ strong activation of forward reaction in the light at concentrations above 0.1 mM Rhodobacter capsulatus
K+ strong activation of forward reaction, i.e. the reduction of NADP+ by NADH, in the light at concentrations of approx. 50-80 mM, half-maximal activation at 10 mM Rhodobacter capsulatus
Mg2+ required for catalytic activity Rhodobacter capsulatus
Mg2+ strong activation of forward reaction, i.e. the reduction of NADP+ by NADH, in the light at concentrations above 0.1 mM Rhodobacter capsulatus
Na+ strong activation of forward reaction, i.e. the reduction of NADP+ by NADH, in the light at concentrations of approx. 50-80 mM, half-maximal activation at 10 mM Rhodobacter capsulatus

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
formerly Rhodopseudomonas capsulata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + thio-NADP+
-
Rhodobacter capsulatus NAD+ + thio-NADPH
-
r

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Rhodobacter capsulatus
NADH
-
Rhodobacter capsulatus
NADP+
-
Rhodobacter capsulatus
NADPH
-
Rhodobacter capsulatus