Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenylyl (beta,gamma-methylene)-diphosphonate | 50% inhibition at 5 mM | Pseudomonas denitrificans (nom. rej.) | |
adenylyl-imidodiphosphate | 50% inhibition at 2 mM | Pseudomonas denitrificans (nom. rej.) | |
ADP | 50% inhibition at 8 mM | Pseudomonas denitrificans (nom. rej.) | |
AMP | 15% inhibition at 10 mM | Pseudomonas denitrificans (nom. rej.) | |
Cu2+ | 50% inhibition at 0.002 mM | Pseudomonas denitrificans (nom. rej.) | |
diphosphate | 50% inhibition at 5 mM | Pseudomonas denitrificans (nom. rej.) | |
Fe2+ | 50% inhibition at 0.05 mM | Pseudomonas denitrificans (nom. rej.) | |
N-ethylmaleimide | 50% inhibition at 0.005 mM | Pseudomonas denitrificans (nom. rej.) | |
Ni2+ | 50% inhibition at 0.003 mM | Pseudomonas denitrificans (nom. rej.) | |
Zn2+ | 50% inhibition at 0.0005 mM | Pseudomonas denitrificans (nom. rej.) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000085 | - |
hydrogenobyrinic acid a,c-diamide | pH 8.0, 30°C | Pseudomonas denitrificans (nom. rej.) | |
0.0042 | - |
Co2+ | pH 8.0, 30°C | Pseudomonas denitrificans (nom. rej.) | |
0.22 | - |
ATP | pH 8.0, 30°C | Pseudomonas denitrificans (nom. rej.) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | ATP binding cation | Pseudomonas denitrificans (nom. rej.) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
1 * 140000, CobN + 1 * 38000, namely CobS + 1 * 80000, namely CobT, gel filtration, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
80000 | - |
1 * 140000, CobN + 1 * 38000, namely CobS + 1 * 80000, namely CobT, gel filtration, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
140000 | - |
1 * 140000, CobN + 1 * 38000, namely CobS + 1 * 80000, namely CobT, gel filtration, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
258000 | - |
gel filtration | Pseudomonas denitrificans (nom. rej.) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydrogenobyrinic acid a,c-diamide + ATP + Co2+ | Pseudomonas denitrificans (nom. rej.) | - |
ADP + phosphate + cob(II)yrinic acid a,c-diamide + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas denitrificans (nom. rej.) | P29933 AND P29934 AND Q9HZQ3 AND P29929 | - |
- |
Purification (Comment) | Organism |
---|---|
purification includes: Mono Q HR 10/10, Penyl-Superose and Mono Q HR 5/5 chromatographies for CobN subunit, Mono Q HR 10/10, Phenyl-Superose and AGATP for CobST subunit | Pseudomonas denitrificans (nom. rej.) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
0.0057 units for CobST subunit, 1 unit of CobN is defined as the amount of CobST necessary to reconstruct 1 unit of the entire complex. 0.006 for CobN subunit, 1 unit of CobN is defined as the amount of CobN necessary to reconstruct 1 unit of the entire complex | Pseudomonas denitrificans (nom. rej.) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + hydrogenobyrinic acid a,c-diamide + Co2+ | - |
Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + cob(II)yrinic acid a,c-diamide + H+ | - |
? | |
CTP + hydrogenobyrinic acid a,c-diamide + Co2+ | 45% of activity with respect to ATP | Pseudomonas denitrificans (nom. rej.) | CDP + phosphate + cob(II)yrinic acid a,c-diamide + H+ | - |
? | |
dATP + hydrogenobyrinic acid a,c-diamide + Co2+ | 65% of activity with respect to ATP | Pseudomonas denitrificans (nom. rej.) | dADP + phosphate + cob(II)yrinic acid a,c-diamide + H+ | - |
? | |
hydrogenobyrinic acid a,c-diamide + ATP + Co2+ | - |
Pseudomonas denitrificans (nom. rej.) | ADP + phosphate + cob(II)yrinic acid a,c-diamide + H+ | - |
? | |
ITP + hydrogenobyrinic acid a,c-diamide + Co2+ | 5% of activity with respect to ATP | Pseudomonas denitrificans (nom. rej.) | IDP + phosphate + cob(II)yrinic acid a,c-diamide + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 140000, CobN + 1 * 38000, namely CobS + 1 * 80000, namely CobT, gel filtration, SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
Synonyms | Comment | Organism |
---|---|---|
cobalt chelatase | - |
Pseudomonas denitrificans (nom. rej.) |
cobaltochelatase | - |
Pseudomonas denitrificans (nom. rej.) |
CobN-CobST | - |
Pseudomonas denitrificans (nom. rej.) |
CobNST | - |
Pseudomonas denitrificans (nom. rej.) |
gene cobN/gene cobS cobaltochelatase | - |
Pseudomonas denitrificans (nom. rej.) |
holocobalamin synthase | - |
Pseudomonas denitrificans (nom. rej.) |
hydrogenobyrinic acid a,c-diamide cobaltochelatase | - |
Pseudomonas denitrificans (nom. rej.) |