Literature summary for 6.6.1.1 extracted from

Chen, X.; Pu, H.; Fang, Y.; Wang, X.; Zhao, S.; Lin, Y.; Zhang, M.; Dai, H.E.; Gong, W.; Liu, L.
Crystal structure of the catalytic subunit of magnesium chelatase (2015), Nat. Plants, 1, 15125 .

Search for more literature for 6.6.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Synechocystis sp. PCC 6803

Crystallization (Commentary)

Crystallization (Comment)	Organism
vapor diffusion method, using 0.85 M sodium citrate, pH 7.0 and 2 mM dithiothreitol	Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + protoporphyrin IX + Mg2+ + H2O	Synechocystis sp. PCC 6803	-	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Synechocystis sp. PCC 6803	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography and Superdex 200 gel filtration	Synechocystis sp. PCC 6803

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + protoporphyrin IX + Mg2+ + H2O	-	Synechocystis sp. PCC 6803	ADP + phosphate + Mg-protoporphyrin IX + 2 H+	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 150000, subunit ChlH, SDS-PAGE	Synechocystis sp. PCC 6803

Synonyms

Synonyms	Comment	Organism
ChlH	catalytic subunit	Synechocystis sp. PCC 6803
MGCH	-	Synechocystis sp. PCC 6803

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Synechocystis sp. PCC 6803

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Release 2023.1 (January 2023)