Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rhodobacter capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Urea | 20% inhibition with 100 mM, 50% inhibition with 250 mM, 90% inhibition with 800 mM | Rhodobacter capsulatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00123 | - |
protoporphyrin IX | pH 8.0, 30ΒΊC | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protoporphyrin IX + Mg2+ + H2O | Rhodobacter capsulatus | - |
ADP + phosphate + Mg-protoporphyrin IX + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
cation exchange chromatography | Rhodobacter capsulatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ | subunits H, I and D from bchH, bchI and bchD genes combine to form the enzyme complex, the complex is active only when the three proteins are present | Rhodobacter capsulatus | |
ATP + protoporphyrin IX + Mg2+ + H2O = ADP + phosphate + Mg-protoporphyrin IX + 2 H+ | this is the first committed step of chlorophyll biosynthesis and is a branchpoint of two major routes in the tetrapyrrole pathway | Rhodobacter capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + protoporphyrin IX + Mg2+ + H2O | - |
Rhodobacter capsulatus | ADP + phosphate + Mg-protoporphyrin IX + H+ | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rhodobacter capsulatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
inactive below pH 6.0 and above pH 10.5 | Rhodobacter capsulatus |