BRENDA - Enzyme Database
show all sequences of 6.5.1.B3

Characterization of a heat-stable enzyme possessing GTP-dependent RNA ligase activity from a hyperthermophilic archaeon, Pyrococcus furiosus

Kanai, A.; Sato, A.; Fukuda, Y.; Okada, K.; Matsuda, T.; Sakamoto, T.; Muto, Y.; Yokoyama, S.; Kawai, G.; Tomita, M.; RNA 15, 420-431 (2009)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
GTP
the activity is dependent on GTP. The GTP structure is important but GTP hydrolysis is not required for the reaction, and GTPgammaS enhances the tRNA ligation activity. GTP is specific and indispensable for the tRNA ligation reaction. ATP, CTP, UTP, dATP, and dGTP cannot substitute for GTP. Neither GDP nor GMP enhance the ligation activity
Pyrococcus furiosus
Cloned(Commentary)
Cloned (Commentary)
Organism
overexpression of recombinant C-terminal His-tagged protein
Pyrococcus furiosus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
Pyrococcus furiosus
-
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pyrococcus furiosus
Q8U4Q3
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pyrococcus furiosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
-
723755
Pyrococcus furiosus
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
-
-
-
?
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
synthetic tRNA halves bearing 2',3'-cyclic phosphate and 5'-OH termini are substrates for the ligation reaction in vitro. The activity is dependent on GTP. The GTP structure is important but GTP hydrolysis is not required for the reaction, and GTPgammaS enhances the tRNA ligation activity. GTP is specific and indispensable for the tRNA ligation reaction. ATP, CTP, UTP, dATP, and dGTP cannot substitute for GTP. Neither GDP nor GMP enhance the ligation activity. The enzyme cleaves a 2',3'-cyclic phosphate group to produce a 2'-phosphate group at the 3' terminus of an RNA oligomer
723755
Pyrococcus furiosus
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
the ligation product is unstable during in vitro reaction
-
-
?
Synonyms
Synonyms
Commentary
Organism
GTP-dependent RNA ligase
-
Pyrococcus furiosus
PF0027
-
Pyrococcus furiosus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Pyrococcus furiosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Pyrococcus furiosus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
GTP
the activity is dependent on GTP. The GTP structure is important but GTP hydrolysis is not required for the reaction, and GTPgammaS enhances the tRNA ligation activity. GTP is specific and indispensable for the tRNA ligation reaction. ATP, CTP, UTP, dATP, and dGTP cannot substitute for GTP. Neither GDP nor GMP enhance the ligation activity
Pyrococcus furiosus
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression of recombinant C-terminal His-tagged protein
Pyrococcus furiosus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
Pyrococcus furiosus
-
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
-
723755
Pyrococcus furiosus
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
-
-
-
?
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end
synthetic tRNA halves bearing 2',3'-cyclic phosphate and 5'-OH termini are substrates for the ligation reaction in vitro. The activity is dependent on GTP. The GTP structure is important but GTP hydrolysis is not required for the reaction, and GTPgammaS enhances the tRNA ligation activity. GTP is specific and indispensable for the tRNA ligation reaction. ATP, CTP, UTP, dATP, and dGTP cannot substitute for GTP. Neither GDP nor GMP enhance the ligation activity. The enzyme cleaves a 2',3'-cyclic phosphate group to produce a 2'-phosphate group at the 3' terminus of an RNA oligomer
723755
Pyrococcus furiosus
a mature tRNA molecule containing a 2'-5'-phosphodiester bond
the ligation product is unstable during in vitro reaction
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Pyrococcus furiosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
assay at
Pyrococcus furiosus
Other publictions for EC 6.5.1.B3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723755
Kanai
Characterization of a heat-sta ...
Pyrococcus furiosus
RNA
15
420-431
2009
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721179
Gao
The structure of Pyrococcus ho ...
Pyrococcus horikoshii, Pyrococcus horikoshii OT-3
Acta Crystallogr. Sect. F
62
1196-1200
2006
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