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Literature summary for 6.5.1.8 extracted from

  • Remus, B.S.; Goldgur, Y.; Shuman, S.
    Structural basis for the GTP specificity of the RNA kinase domain of fungal tRNA ligase (2017), Nucleic Acids Res., 45, 12945-12953 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus(DE3) cells Candida albicans

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with GDP and Mg2+, sitting drop vapor diffusion method, using 0.2 M sodium dihydrogen phosphate, 20% (w/v) PEG3350 Candida albicans

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O Candida albicans
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP Candida albicans
-
 (ribonucleotide)n+m + GMP + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography and gel filtration Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O
-
 Candida albicans (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 2-aminopurine-riboside-5'-triphosphate about 20% activity with 0.1 mM 2-aminopurine triphosphate compared to GTP Candida albicans (ribonucleotide)n+m + 2-aminopurine-riboside-5'-monophosphate + diphosphate
-
 ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 2-mercaptopurine-riboside-5'-triphosphate about 25% activity with 0.1 mM 2-mercaptopurine triphosphate compared to GTP Candida albicans (ribonucleotide)n+m + 2-mercaptopurine-riboside-5'-monophosphate + diphosphate
-
 ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 6-chloropurine-riboside-5'-triphosphate about 1% activity with 0.1 mM 6-chloropurine triphosphate compared to GTP Candida albicans (ribonucleotide)n+m + 6-chloropurine-riboside-5'-monophosphate + diphosphate
-
 ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + 6-O-methylguanosine triphosphate about 45% activity with 0.1 mM 6-O-methyl triphosphate compared to GTP Candida albicans (ribonucleotide)n+m + 6-O-methylguanosine phosphate + diphosphate
-
 ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + ATP about 22% activity with 0.1 mM ATP compared to GTP Candida albicans (ribonucleotide)n+m + AMP + diphosphate
-
 ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + CTP about 18% activity with 0.1 mM CTP compared to GTP Candida albicans (ribonucleotide)n+m + CMP + diphosphate
-
 ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
-
 Candida albicans (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP 100% activity with 0.01 mM GTP Candida albicans (ribonucleotide)n+m + GMP + diphosphate
-
 ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + ITP about 75% activity with 0.1 mM ITP compared to GTP Candida albicans (ribonucleotide)n+m + IMP + diphosphate
-
 ?
(ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + UTP about 85% activity with 0.1 mM UTP compared to GTP Candida albicans (ribonucleotide)n+m + UMP + diphosphate
-
 ?
additional information ATP and CTP are at least 3 orders of magnitude less effective as phosphate donors compared to GTP Candida albicans ?
-
 ?

Synonyms

Synonyms Comment Organism
Trl1
-
 Candida albicans

Cofactor

Cofactor Comment Organism Structure
GTP
-
 Candida albicans

General Information

General Information Comment Organism
physiological function fungal tRNA ligase Trl1 is an essential enzyme that repairs RNA breaks with 2',3'-cyclic-PO4 and 5'-OH ends inflicted during tRNA splicing and noncanonical mRNA splicing in the fungal unfolded protein response Candida albicans