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Literature summary for 6.5.1.4 extracted from

  • Vicente, O.; Filipowicz, W.
    Purification of RNA 3'-terminal phosphate cyclase from HeLa cells (1988), Eur. J. Biochem., 17, 431-439.
    View publication on PubMed
Search for more literature for 6.5.1.4

General Stability

General Stability Organism
not affected by several cycles of freezing and thawing Homo sapiens
resistant to protease digestion Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
iodoacetamide
-
 Homo sapiens
NEM
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 ATP
-
 Homo sapiens
0.006
-
 ATPgammaS ATP Homo sapiens
0.2
-
 GTP
-
 Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ only 5% of the activation by Mg2+ Homo sapiens
K+ optimal concentration: 150-200 mM Homo sapiens
Mg2+ required Homo sapiens
Mg2+ maximal activity at 4-5 mM Homo sapiens
Mn2+ only 5% of the activation by Mg2+ Homo sapiens
Na+ optimal concentration: 150-200 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000 40000 glycerol density gradient sedimentation, gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + [RNA]-3'-(3'-phospho-ribonucleoside) = AMP + diphosphate + [RNA]-3'-(2',3'-cyclophospho)-ribonucleoside formation of a covalent cyclase-AMP intermediate Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, stable for at least 6 months Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RNA 3'-terminal-phosphate no activity with dATP Homo sapiens AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
ATPgammaS + RNA 3'-terminal-phosphate
-
 Homo sapiens AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
CTP + RNA 3'-terminal-phosphate
-
 Homo sapiens CMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
GTP + RNA 3'-terminal-phosphate
-
 Homo sapiens GMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?
UTP + RNA 3'-terminal-phosphate
-
 Homo sapiens UMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000-40000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 similar rates of reaction at 25°C, 30°C, and 37°C Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
 37 0°C: 25% of maximal activity, 12°C: 60% of maximal activity, 25-37°C: maximal activity Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 5 min, 55% loss of activity Homo sapiens
50
-
 5 min, 95% loss of activity Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9
-
 Homo sapiens