Any feedback?
Literature summary for 6.5.1.4 extracted from
- The enzymatic conversion of 3'-phosphate terminated RNA chains to 2',3'-cyclic phosphate derivatives (1985), J. Biol. Chem., 260, 6088-6097.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NEM | - |
Homo sapiens |  |
| SDS | - |
Homo sapiens | |
| Sodium phosphate | - |
Homo sapiens | |
| Vanadyl-ribonucleoside complex | - |
Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
ATP | - |
Homo sapiens | |
| 0.006 | - |
ATPgammaS | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens | |
| Mg2+ | Km: 2.5 mM | Homo sapiens | |
| Mn2+ | cannot replace Mg2+ in activation | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | 40000 | glycerol density gradient sedimentation, gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| HeLa cell | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.021 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, stable for at least 3 months | Homo sapiens |
| 4°C, 50% loss of activity after 3 days | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + RNA 3'-terminal-phosphate | ir | Homo sapiens | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATP + RNA 3'-terminal-phosphate | (Up)10Gp is cyclized at a faster rate than tRNAApGp or AUGp | Homo sapiens | AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| ATPgammaS + RNA 3'-terminal-phosphate | ir | Homo sapiens | AMP + thiodiphosphate + RNA terminal-2',3'-cyclic-phosphate | - |
? | |
| additional information | ATP/diphosphate exchange reaction not detected | Homo sapiens | ? | - |
? | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
