Literature summary for 6.5.1.3 extracted from

Sillero, M.A.; de Diego, A.; Tavares, J.E.; Silva, J.A.; Perez-Zuniga, F.J.; Sillero, A.
Synthesis of ATP derivatives of compounds of the mevalonate pathway (isopentenyl di- and triphosphate; geranyl di- and triphosphate, farnesyl di- and triphosphate, and dimethylallyl diphosphate) catalyzed by T4 RNA ligase, T4 DNA ligase and other ligases (2009), Biochem. Pharmacol., 78, 335-343.

Search for more literature for 6.5.1.3

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.07	-	(2E,6E)-farnesyl triphosphate	-	Tequatrovirus T4
0.23	-	geranyl triphosphate	-	Tequatrovirus T4
0.24	-	(2E,6E)-farnesyl diphosphate	-	Tequatrovirus T4
0.29	-	geranyl diphosphate	-	Tequatrovirus T4
0.86	-	dimethylallyl diphosphate	-	Tequatrovirus T4
3.6	-	isopentenyl triphosphate	-	Tequatrovirus T4
4.9	-	isopentenyl diphosphate	-	Tequatrovirus T4

Organism

Organism	UniProt	Comment	Textmining
Tequatrovirus T4	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0034	-	with geranyl diphosphate as substrate	Tequatrovirus T4
0.0037	-	with dimethylallyl diphosphate as substrate	Tequatrovirus T4
0.004	-	with (2E,6E)-farnesyl diphosphate as substrate	Tequatrovirus T4
0.0062	-	with isopentenyl diphosphate as substrate	Tequatrovirus T4
0.016	-	with (2E,6E)-farnesyl triphosphate as substrate	Tequatrovirus T4
0.0166	-	with geranyl triphosphate as substrate	Tequatrovirus T4
0.0335	-	with isopentenyl triphosphate as substrate	Tequatrovirus T4

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (2E,6E)-farnesyl diphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	(2E,6E)-farnesyl-triphospho(5')adenosine + diphosphate	-	ir
ATP + (2E,6E)-farnesyl triphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	(2E,6E)-farnesyl-tetraphospho(5')adenosine + diphosphate	-	ir
ATP + dimethylallyl diphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	dimethylallyl-triphospho(5')adenosine + diphosphate	-	ir
ATP + geranyl diphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	geranyl-triphospho(5')adenosine + diphosphate	-	ir
ATP + geranyl triphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	geranyl-tetraphospho(5')adenosine + diphosphate	-	ir
ATP + isopentenyl diphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	adenosine 5'-isopentenyl triphosphate + diphosphate	-	ir
ATP + isopentenyl triphosphate	assay contains pyruvate kinase, phosphoenolpyruvate and pyrophosphatase to recover ATP	Tequatrovirus T4	isopentenyl-tetraphospho(5')adenosine + diphosphate	-	ir

Synonyms

Synonyms	Comment	Organism
RNA ligase	-	Tequatrovirus T4
T4 RNA ligase	-	Tequatrovirus T4

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.9	-	isopentenyl diphosphate	-	Tequatrovirus T4
3.1	-	dimethylallyl diphosphate	-	Tequatrovirus T4
6.7	-	isopentenyl triphosphate	-	Tequatrovirus T4
8.6	-	geranyl diphosphate	-	Tequatrovirus T4
11.6	-	(2E,6E)-farnesyl triphosphate	-	Tequatrovirus T4
12.1	-	(2E,6E)-farnesyl diphosphate	-	Tequatrovirus T4
52.2	-	geranyl triphosphate	-	Tequatrovirus T4

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
2.5	-	geranyl diphosphate	-	Tequatrovirus T4
2.7	-	dimethylallyl diphosphate	-	Tequatrovirus T4
2.9	-	(2E,6E)-farnesyl diphosphate	-	Tequatrovirus T4
4.5	-	isopentenyl diphosphate	-	Tequatrovirus T4
12	-	geranyl triphosphate	-	Tequatrovirus T4
24	-	isopentenyl triphosphate	-	Tequatrovirus T4
166	-	(2E,6E)-farnesyl triphosphate	-	Tequatrovirus T4

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)