Protein Variants | Comment | Organism |
---|---|---|
H250A | pRNA circularization by the mutant enzyme is 6% of the wild-type rate | Tequatrovirus T4 |
K117A | mutation is lethal in vivo | Tequatrovirus T4 |
L104A | L104A strain is temperature sensitive, no growth at 37°C | Tequatrovirus T4 |
L104A | pRNA circularization by the mutant enzyme is 6% of the wild-type rate | Tequatrovirus T4 |
additional information | S121A, S124A, and H250 strains grow at all temperatures | Tequatrovirus T4 |
S103A | pRNA circularization by the mutant enzyme is 5% of the wild-type rate | Tequatrovirus T4 |
S103A | S103A strain is temperature sensitive, no growth at 37°C | Tequatrovirus T4 |
S118A | S118 strain is temperature sensitive, no growth at 37°C | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (ribonucleotide)n + (ribonucleotide)m | Rnl1 has an inherent specificity for sealing tRNA with a break in the anticodon loop. The tRNA specificity is imparted by the C domain | Tequatrovirus T4 | AMP + diphosphate + (ribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNA ligase 1 | - |
Tequatrovirus T4 |
Rnl1 | - |
Tequatrovirus T4 |