Protein Variants | Comment | Organism |
---|---|---|
D135A | about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 4% of the adenylyltransferase activity observed with wild-type enzyme | Tequatrovirus T4 |
D292A | no ligation activity | Tequatrovirus T4 |
D292E | about 10% of the ligation activity of wild-type enzyme | Tequatrovirus T4 |
D308A | about 70% of the ligation activity of wild-type enzyme | Tequatrovirus T4 |
E139A | about as active as wild-type enzyme in RNA ligation, mutant displays near wild-type adenylyltransferase activity | Tequatrovirus T4 |
E295A | about as active as wild-type enzyme in RNA ligation | Tequatrovirus T4 |
E296A | about 10% of the ligation activity of wild-type enzyme | Tequatrovirus T4 |
E299A | about 80% of the ligation activity of wild-type enzyme | Tequatrovirus T4 |
E29A | about 90% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity | Tequatrovirus T4 |
E300A | about 50% of the ligation activity of wild-type enzyme | Tequatrovirus T4 |
E63A | about 80% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity | Tequatrovirus T4 |
K107A | about as active as wild-type enzyme in RNA ligation, mutant displays near wild-type adenylyltransferase activity | Tequatrovirus T4 |
K273A | about as active as wild-type enzyme in RNA ligation | Tequatrovirus T4 |
K314A | about as active as wild-type enzyme in RNA ligation | Tequatrovirus T4 |
K315A | about 90% of the ligation activity of wild-type enzyme | Tequatrovirus T4 |
K319A | about as active as wild-type enzyme in RNA ligation | Tequatrovirus T4 |
K54A | about 15% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity | Tequatrovirus T4 |
N309A | about 70% of the ligation activity of wild-type enzyme | Tequatrovirus T4 |
Q106A | about 20% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity | Tequatrovirus T4 |
R155A | about 35% of the ligation activity of wild-type enzyme, mutant enzyme displays 16% of the adenylyltransferase activity observed with wild-type enzyme | Tequatrovirus T4 |
R221A | about 60% of the ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity | Tequatrovirus T4 |
R266A | no ligation activity | Tequatrovirus T4 |
R266K | about 50% of the ligation activity of wild-type enzyme | Tequatrovirus T4 |
R266Q | no ligation activity | Tequatrovirus T4 |
R33A | no ligation activity, mutant enzyme displays 3% of the adenylyltransferase activity observed with wild-type enzyme | Tequatrovirus T4 |
S170A | about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 8% of the adenylyltransferase activity observed with wild-type enzyme | Tequatrovirus T4 |
S272A | about 50% of the ligation activity of wild-type enzyme | Tequatrovirus T4 |
S292N | about 25% of the ligation activity of wild-type enzyme | Tequatrovirus T4 |
W329A | about as active as wild-type enzyme in RNA ligation | Tequatrovirus T4 |
Y136A | about 95% of the ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity | Tequatrovirus T4 |
Y5A | about 10% of the RNA ligation activity of wild-type enzyme, mutant enzyme displays 39% of the adenylyltransferase activity observed with wild-type enzyme | Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mg2+ or Mn2+ required | Tequatrovirus T4 | |
Mn2+ | Mg2+ or Mn2+ required | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (ribonucleotide)n + (ribonucleotide)m | neither dATP, GTP, CTP, nor UTP can substitute for ATP. RNA ligation at a 3'-OH/5'-PO4 nick in a double-stranded RNA or an RNA-DNA hybrid | Tequatrovirus T4 | AMP + diphosphate + (ribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RNA ligase 2 | - |
Tequatrovirus T4 |
Rnl2 | - |
Tequatrovirus T4 |