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Literature summary for 6.5.1.3 extracted from

  • Nandakumar, J.; Ho, C.K.; Lima, C.D.; Shuman, S.
    RNA substrate specificity and structure-guided mutational analysis of bacteriophage T4 RNA ligase 2 (2004), J. Biol. Chem., 279, 31337-31347.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D135A about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 4% of the adenylyltransferase activity observed with wild-type enzyme Tequatrovirus T4
D292A no ligation activity Tequatrovirus T4
D292E about 10% of the ligation activity of wild-type enzyme Tequatrovirus T4
D308A about 70% of the ligation activity of wild-type enzyme Tequatrovirus T4
E139A about as active as wild-type enzyme in RNA ligation, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
E295A about as active as wild-type enzyme in RNA ligation Tequatrovirus T4
E296A about 10% of the ligation activity of wild-type enzyme Tequatrovirus T4
E299A about 80% of the ligation activity of wild-type enzyme Tequatrovirus T4
E29A about 90% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
E300A about 50% of the ligation activity of wild-type enzyme Tequatrovirus T4
E63A about 80% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
K107A about as active as wild-type enzyme in RNA ligation, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
K273A about as active as wild-type enzyme in RNA ligation Tequatrovirus T4
K314A about as active as wild-type enzyme in RNA ligation Tequatrovirus T4
K315A about 90% of the ligation activity of wild-type enzyme Tequatrovirus T4
K319A about as active as wild-type enzyme in RNA ligation Tequatrovirus T4
K54A about 15% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
N309A about 70% of the ligation activity of wild-type enzyme Tequatrovirus T4
Q106A about 20% of the RNA ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
R155A about 35% of the ligation activity of wild-type enzyme, mutant enzyme displays 16% of the adenylyltransferase activity observed with wild-type enzyme Tequatrovirus T4
R221A about 60% of the ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
R266A no ligation activity Tequatrovirus T4
R266K about 50% of the ligation activity of wild-type enzyme Tequatrovirus T4
R266Q no ligation activity Tequatrovirus T4
R33A no ligation activity, mutant enzyme displays 3% of the adenylyltransferase activity observed with wild-type enzyme Tequatrovirus T4
S170A about 5% of the ligation activity of wild-type enzyme, mutant enzyme displays 8% of the adenylyltransferase activity observed with wild-type enzyme Tequatrovirus T4
S272A about 50% of the ligation activity of wild-type enzyme Tequatrovirus T4
S292N about 25% of the ligation activity of wild-type enzyme Tequatrovirus T4
W329A about as active as wild-type enzyme in RNA ligation Tequatrovirus T4
Y136A about 95% of the ligation activity of wild-type enzyme, mutant displays near wild-type adenylyltransferase activity Tequatrovirus T4
Y5A about 10% of the RNA ligation activity of wild-type enzyme, mutant enzyme displays 39% of the adenylyltransferase activity observed with wild-type enzyme Tequatrovirus T4

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ Mg2+ or Mn2+ required Tequatrovirus T4
Mn2+ Mg2+ or Mn2+ required Tequatrovirus T4

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m neither dATP, GTP, CTP, nor UTP can substitute for ATP. RNA ligation at a 3'-OH/5'-PO4 nick in a double-stranded RNA or an RNA-DNA hybrid Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
?

Synonyms

Synonyms Comment Organism
RNA ligase 2
-
Tequatrovirus T4
Rnl2
-
Tequatrovirus T4