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Literature summary for 6.5.1.3 extracted from

  • Yin, S.; Kiong Ho, C.; Miller, E.S.; Shuman, S.
    Characterization of bacteriophage KVP40 and T4 RNA ligase 2 (2004), Virology, 319, 141-151.
    View publication on PubMed
Search for more literature for 6.5.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a His10-tagged fusion protein Vibrio phage KVP40

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
 adsorption to Ni-agarose and step-elution with imidazole Vibrio phage KVP40

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m Tequatrovirus T4
-
 AMP + diphosphate + (ribonucleotide)n+m
-
 ?
ATP + (ribonucleotide)n + (ribonucleotide)m Vibrio phage KVP40 catalyzes RNA end-joining AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-
Vibrio phage KVP40
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Vibrio phage KVP40

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Tequatrovirus T4 AMP + diphosphate + (ribonucleotide)n+m
-
 ?
ATP + (ribonucleotide)n + (ribonucleotide)m
-
 Vibrio phage KVP40 AMP + diphosphate + (ribonucleotide)n+m
-
 ?
ATP + (ribonucleotide)n + (ribonucleotide)m catalyzes RNA end-joining Vibrio phage KVP40 AMP + diphosphate + (ribonucleotide)n+m
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 42000, SDS-PAGE Vibrio phage KVP40

Synonyms

Synonyms Comment Organism
phage Rnl2
-
 Vibrio phage KVP40
RNA ligase (ATP)
-
 Vibrio phage KVP40
Rnl2
-
 Tequatrovirus T4
Rnl2
-
 Vibrio phage KVP40
T4 RNA ligase 2
-
 Tequatrovirus T4

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 adenylyltransferase activity Vibrio phage KVP40

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 activity is virtually nil at pH below 5.0 or above 9.5 Vibrio phage KVP40