Cloned (Comment) | Organism |
---|---|
RnlA gene overexpressed in Escherichia coli BL21 | Rhodothermus phage RM378 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | - |
Rhodothermus phage RM378 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimum activity at 5 mM | Rhodothermus phage RM378 | |
Mn2+ | optimum activity at 1 mM | Rhodothermus phage RM378 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51300 | - |
calculated from the putative RNA ligase 1 gene | Rhodothermus phage RM378 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (ribonucleotide)n + (ribonucleotide)m | Rhodothermus phage RM378 | - |
AMP + diphosphate + (ribonucleotide)n+m | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodothermus phage RM378 | - |
thermophilic bacteriophage that infects the thermophilic eubacterium Rhodothermus marinus | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Rhodothermus phage RM378 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (ribonucleotide)n + (ribonucleotide)m | - |
Rhodothermus phage RM378 | AMP + diphosphate + (ribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bacteriophage RNA ligase | - |
Rhodothermus phage RM378 |
RM378 RNA ligase | - |
Rhodothermus phage RM378 |
RnlA | - |
Rhodothermus phage RM378 |
thermostable RNA ligase 1 | - |
Rhodothermus phage RM378 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
64 | - |
- |
Rhodothermus phage RM378 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | relatively stable at 60°C with a half-life of 10 h, activity increases after incubation, not stable at temperatures. Enzyme starts to lose activity rapidly, no activity at all after 1 h at 80°C, loses all of its activity after 5 min at 90°C | Rhodothermus phage RM378 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
Tris buffer | Rhodothermus phage RM378 |
7 | - |
MOPS buffer | Rhodothermus phage RM378 |