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Literature summary for 6.5.1.2 extracted from

  • Shrivastava, N.; Nag, J.K.; Misra-Bhattacharya, S.
    Molecular characterization of NAD+-dependent DNA ligase from Wolbachia endosymbiont of lymphatic filarial parasite Brugia malayi (2012), PLoS ONE, 7, e41113.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpressed as a His-tagged fusion protein in Escherichia coli Wolbachia endosymbiont of Brugia malayi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000193
-
deoxyribonucleotide pH 8, 25°C Wolbachia endosymbiont of Brugia malayi
0.0048
-
NAD+ pH 8, 25°C Wolbachia endosymbiont of Brugia malayi

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ no activity observed in the presence of Wolbachia endosymbiont of Brugia malayi
KCl enzymatic activity increased in the presence of monovalent cations, maximal activity at 25 mM NaCl Wolbachia endosymbiont of Brugia malayi
MgCl2 highest activity: 10 mM MgCl2 Wolbachia endosymbiont of Brugia malayi
NaCl enzymatic activity increased in the presence of monovalent cations, maximal activity at 5 mM NaCl Wolbachia endosymbiont of Brugia malayi
NH4Cl enzymatic activity increased in the presence of monovalent cations, maximal activity at 10 mM NaCl Wolbachia endosymbiont of Brugia malayi
Ni2+ no activity observed in the presence of Wolbachia endosymbiont of Brugia malayi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
SDS-PAGE, recombinant protein Wolbachia endosymbiont of Brugia malayi

Organism

Organism UniProt Comment Textmining
Wolbachia endosymbiont of Brugia malayi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Wolbachia endosymbiont of Brugia malayi

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + deoxyribonucleotide
-
Wolbachia endosymbiont of Brugia malayi AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?

Synonyms

Synonyms Comment Organism
wBm-LigA
-
Wolbachia endosymbiont of Brugia malayi

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Wolbachia endosymbiont of Brugia malayi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Wolbachia endosymbiont of Brugia malayi

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5
-
Wolbachia endosymbiont of Brugia malayi

Expression

Organism Comment Expression
Wolbachia endosymbiont of Brugia malayi high enzyme expression is observed in Wolbachia within Brugia malayi microfilariae and female adult parasites along the hypodermal chords and in the gravid portion up