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Literature summary for 6.5.1.2 extracted from

  • Shapiro, A.B.; Eakin, A.E.; Walkup, G.K.; Rivin, O.
    A high-throughput fluorescence resonance energy transfer-based assay for DNA ligase (2011), J. Biomol. Screen., 16, 486-493.
    View publication on PubMed
Search for more literature for 6.5.1.2

Activating Compound

Activating Compound Comment Organism Structure
(NH4)2SO4 with 30 mM (NH4)2SO4, the activity is maximal Haemophilus influenzae
KCl activation by KCl is observed at (NH4)2SO4 concentrations below 30 mM, in the absence of (NH4)2SO4 ligase activity is activated by KCl over 0-100 mM, and at 100 mM KCl, further activation occurs at up to 12 mM (NH4)2SO4 Haemophilus influenzae

Cloned(Commentary)

Cloned (Comment) Organism
-
 Haemophilus influenzae

Inhibitors

Inhibitors Comment Organism Structure
(NH4)2SO4 (NH4)2SO4 concentrations higher than 30 mM are inhibitory. At 100 mM KCl, concentrations of (NH4)2SO4 above 12 mM are inhibitory Haemophilus influenzae
1,N6-etheno-NAD+
-
 Haemophilus influenzae
KCl inhibition by KCl occurs at greater than 30 mM (NH4)2SO Haemophilus influenzae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required for activity, maximal activity occurs with 10-20 mM MgCl2 in the presence of 30 mM each of KCl and (NH4)2SO4 Haemophilus influenzae

Organism

Organism UniProt Comment Textmining
Haemophilus influenzae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haemophilus influenzae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Haemophilus influenzae AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Synonyms

Synonyms Comment Organism
NAD+-dependent DNA ligase
-
 Haemophilus influenzae

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Haemophilus influenzae

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.054
-
 pH and temperature not specified in the publication Haemophilus influenzae 1,N6-etheno-NAD+