Literature summary for 6.5.1.2 extracted from

Meier, T.I.; Yan, D.; Peery, R.B.; McAllister, K.A.; Zook, C.; Peng, S.B.; Zhao, G.
Identification and characterization of an inhibitor specific to bacterial NAD+-dependent DNA ligases (2008), FEBS J., 275, 5258-5271.

Search for more literature for 6.5.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 (DE3) cells	Streptococcus pneumoniae

Inhibitors

Inhibitors	Comment	Organism	Structure
2,4-diamino-7-dimethylamino-pyrimido[4,5-d]pyrimidine	IC50 of 0.0001 mg/ml, specific inhibitor of NAD+-dependent DNA ligase, inhibits competitively with respect to NAD+ and specifically inhibits enzyme adenylation, but not DNA adenylation or ligation	Streptococcus pneumoniae
methotrexate	IC50 of 0.008 mg/ml	Streptococcus pneumoniae
additional information	not inhibited by trimethoprim	Streptococcus pneumoniae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0021	-	NAD+	-	Streptococcus pneumoniae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
72000	-	SDS-PAGE	Streptococcus pneumoniae
72130	-	calculated from amino acid sequence	Streptococcus pneumoniae

Organism

Organism	UniProt	Comment	Textmining
Streptococcus pneumoniae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Sepharose column chromatography and hydroxyapatite column chromatography	Streptococcus pneumoniae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Streptococcus pneumoniae	AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m	-	?

Subunits

Subunits	Comment	Organism
?	x * 72000, SDS-PAGE	Streptococcus pneumoniae

Synonyms

Synonyms	Comment	Organism
LigA	-	Streptococcus pneumoniae
NAD+-dependent DNA ligase	-	Streptococcus pneumoniae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.1	-	NAD+	pH 8.0, 21°C	Streptococcus pneumoniae

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Streptococcus pneumoniae

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0014	-	2,4-diamino-7-dimethylamino-pyrimido[4,5-d]pyrimidine	-	Streptococcus pneumoniae

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Release 2023.1 (January 2023)