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Literature summary for 6.5.1.2 extracted from
- Characterization of mimivirus NAD+-dependent DNA ligase (2006), Virology, 353, 133-143.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| wild-type and mutant proteins Y36A, D46A, Y49A, D50A, and K113A are produced in Escherichia coli | Mimivirus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D46A | mutant protein displayes only trace sealing activity | Mimivirus |
| D50A | nick sealing activity is 6% of wild-type activity | Mimivirus |
| K113A | mutant protein is inert | Mimivirus |
| additional information | truncated version of MimiLIG (C-DELTA79), composed of residues 1-557, but lacking the C-terminal 79 amino acids that comprise the BRCT domain shows reduced specific activity in nick joining by 75fold without affecting the ligase adenylylation step. The DELTA-RCT mutant of MimiLIG is impaired in sealing at a preadenylylated nick | Mimivirus |
| Y36A | mutant protein displayes only trace sealing activity | Mimivirus |
| Y49A | nick sealing activity is 6% of wild-type activity | Mimivirus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
x * 70000, SDS-PAGE | Mimivirus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mimivirus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| MimiLIG is produced in Escherichia coli as a His10-Smt3 fusion and purified from a soluble bacterial lysate by Ni2+-agarose chromatography | Mimivirus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, SDS-PAGE | Mimivirus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MimiLIG | - |
Mimivirus |
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