Literature summary for 6.5.1.2 extracted from

Lavesa-Curto, M.; Sayer, H.; Bullard, D.; MacDonald, A.; Wilkinson, A.; Smith, A.; Bowater, L.; Hemmings, A.; Bowater, R.P.
Characterization of a temperature-sensitive DNA ligase from Escherichia coli (2004), Microbiology, 150, 4171-4180.

Search for more literature for 6.5.1.2

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
L15F	at 20°C mutant enzyme L15F has 20fold lower ligation activity in vitro, and its activity is reduced at 42°C, resulting in 60fold lower ligation activity than wild-type LigA	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	strain GR501	-
Escherichia coli DSM 3757	-	strain GR501	-

Purification (Commentary)

Purification (Comment)	Organism
-	Escherichia coli

Synonyms

Synonyms	Comment	Organism
LigA	-	Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	mutant enzyme L15F has 20fold lower ligation activity in vitro than wild-type enzyme	Escherichia coli
42	-	mutant enzyme L15F has 60fold lower ligation activity in vitro than wild-type enzyme	Escherichia coli

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Release 2023.1 (January 2023)