Protein Variants | Comment | Organism |
---|---|---|
D117E | 3% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
D117N | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
D138A | 37% of ligase activity of wild-type enzyme | Escherichia coli |
D283A | 57% of ligase activity of wild-type enzyme | Escherichia coli |
D285E | 4% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
D285N | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
E143A | 52% of ligase activity of wild-type enzyme | Escherichia coli |
E173A | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
E173D | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
E173Q | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
G118A | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
G172A | 36% of ligase activity of wild-type enzyme | Escherichia coli |
G286A | 14% of ligase activity of wild-type enzyme | Escherichia coli |
K115Q | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
K115R | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
K290A | 13% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
K314Q | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
K314R | 5% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
L119A | 110% of ligase activity of wild-type enzyme | Escherichia coli |
N198A | 26% of ligase activity of wild-type enzyme | Escherichia coli |
R200A | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
R200K | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
R200Q | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
R208A | 2% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
R208K | 1.2% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
R208Q | less than 0.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
R277A | 1.1% of ligase activity of wild-type enzyme, lethal mutation | Escherichia coli |
R277A | rate of isolated sealing step is 20% of the activity of wild-type enzyme | Escherichia coli |
R277K | 5.6% of ligase activity of wild-type enzyme, themperature-sensitive mutation, rate of isolated sealing step is 4% of the activity of wild-type enzyme | Escherichia coli |
R277Q | 1.8% of ligase activity of wild-type enzyme, lethal mutation, rate of isolated sealing step is 10% of the activity of wild-type enzyme | Escherichia coli |
V288A | 74% of ligase activity of wild-type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Escherichia coli | AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m | - |
? |
Synonyms | Comment | Organism |
---|---|---|
LigA | - |
Escherichia coli |
NAD+-dependent DNA ligase | - |
Escherichia coli |