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Literature summary for 6.5.1.2 extracted from

  • Zhu, H.; Shuman, S.
    Structure-guided mutational analysis of the nucleotidyltransferase domain of Escherichia coli NAD+-dependent DNA ligase (LigA) (2005), J. Biol. Chem., 280, 12137-12144.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D117E 3% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
D117N less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
D138A 37% of ligase activity of wild-type enzyme Escherichia coli
D283A 57% of ligase activity of wild-type enzyme Escherichia coli
D285E 4% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
D285N less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
E143A 52% of ligase activity of wild-type enzyme Escherichia coli
E173A less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
E173D less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
E173Q less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
G118A less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
G172A 36% of ligase activity of wild-type enzyme Escherichia coli
G286A 14% of ligase activity of wild-type enzyme Escherichia coli
K115Q less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
K115R less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
K290A 13% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
K314Q less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
K314R 5% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
L119A 110% of ligase activity of wild-type enzyme Escherichia coli
N198A 26% of ligase activity of wild-type enzyme Escherichia coli
R200A less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
R200K less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
R200Q less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
R208A 2% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
R208K 1.2% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
R208Q less than 0.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
R277A 1.1% of ligase activity of wild-type enzyme, lethal mutation Escherichia coli
R277A rate of isolated sealing step is 20% of the activity of wild-type enzyme Escherichia coli
R277K 5.6% of ligase activity of wild-type enzyme, themperature-sensitive mutation, rate of isolated sealing step is 4% of the activity of wild-type enzyme Escherichia coli
R277Q 1.8% of ligase activity of wild-type enzyme, lethal mutation, rate of isolated sealing step is 10% of the activity of wild-type enzyme Escherichia coli
V288A 74% of ligase activity of wild-type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
Escherichia coli AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
?

Synonyms

Synonyms Comment Organism
LigA
-
Escherichia coli
NAD+-dependent DNA ligase
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Escherichia coli