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Literature summary for 6.5.1.2 extracted from

  • Zauner, G.; Wang, Y.; Lavesa-Curto, M.; MacDonald, A.; Mayes, A.G.; Bowater, R.P.; Butt, J.N.
    Tethered DNA hairpins facilitate electrochemical detection of DNA ligation (2005), Analyst, 130, 345-349.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Escherichia coli
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-
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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electrochemical assay for DNA ligase activity. The assay exploits the properties of DNA hairpins tethered at one terminus to a gold electrode and labelled at the other with a ferrocene group for rapid characterisation of DNA status by cyclic voltammetry. Successful ligation of nicked DNA hairpins is indicated by retention of the ferrocene couple when exposure to DNA ligase is followed by conditions that denature the hairpin Escherichia coli

Synonyms

Synonyms Comment Organism
LigA
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Escherichia coli
NAD+-dependent DNA ligase
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Escherichia coli