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Literature summary for 6.5.1.1 extracted from

  • Williamson, A.; Pedersen, H.
    Recombinant expression and purification of an ATP-dependent DNA ligase from Aliivibrio salmonicida (2014), Protein Expr. Purif., 97, 29-36 .
    View publication on PubMed
Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Star (DE3) pLysS cells Aliivibrio salmonicida

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Aliivibrio salmonicida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m Aliivibrio salmonicida
-
 (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + [DNA ligase]-L-lysine Aliivibrio salmonicida
-
 [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Aliivibrio salmonicida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His Trap column chromatography, Ni-IMAC column chromatography, and Superdex 200 gel filtration Aliivibrio salmonicida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m
-
 Aliivibrio salmonicida (deoxyribonucleotide)n+m + AMP + diphosphate
-
 ?
ATP + [DNA ligase]-L-lysine
-
 Aliivibrio salmonicida [DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 31696, quadrupole-time of flight liquid chromatography-mass spectrometry Aliivibrio salmonicida
? x * 35900, full-length His6-tagged enzyme, calculated from amino acid sequence Aliivibrio salmonicida

Synonyms

Synonyms Comment Organism
ATP-dependent DNA ligase
-
 Aliivibrio salmonicida
Vib-Lig
-
 Aliivibrio salmonicida