Literature summary for 6.5.1.1 extracted from

Pezeshgi Modarres, H.; Dorokhov, B.; Popov, V.; Ravin, N.; Skryabin, K.; Dal Peraro, M.
Understanding and engineering thermostability in DNA ligase from Thermococcus sp. 1519 (2015), Biochemistry, 54, 3076-3085 .

Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta-gami (DE3) cells	Thermococcus sp. 1519

Protein Variants

Protein Variants	Comment	Organism
A287K	the mutant enzyme has a half-life time at 94°C of 20 min	Thermococcus sp. 1519
A387K	the mutant enzyme has a half-life time at 94°C of 15 min	Thermococcus sp. 1519
G304D	the mutant enzyme has a half-life time at 94°C of 25 min	Thermococcus sp. 1519
S364I	the mutant enzyme has a half-life time at 94°C of 20 min	Thermococcus sp. 1519

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	Thermococcus sp. 1519	-	(deoxyribonucleotide)n+m + AMP + diphosphate	-	?
ATP + [DNA ligase]-L-lysine	Thermococcus sp. 1519	-	[DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermococcus sp. 1519	C0LJI8	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography, and Superdex 200 gel filtration	Thermococcus sp. 1519

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m	-	Thermococcus sp. 1519	(deoxyribonucleotide)n+m + AMP + diphosphate	-	?
ATP + [DNA ligase]-L-lysine	-	Thermococcus sp. 1519	[DNA ligase]-N6-(5'-adenylyl)-L-lysine + diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 63380, calculated from amino acid sequence	Thermococcus sp. 1519

Synonyms

Synonyms	Comment	Organism
ATP-dependent DNA ligase	-	Thermococcus sp. 1519
Lig	-	Thermococcus sp. 1519

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
94	-	the unmodified enzyme loses half of its activity upon 8 min incubation at 94°C	Thermococcus sp. 1519

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Release 2023.1 (January 2023)