Application | Comment | Organism |
---|---|---|
molecular biology | inclusion of the C-terminally 6His-tagged PabDBD in the reaction mixture during long-range polymerase chain reaction (PCR) increases the efficacy of amplification and eliminates the inhibitory effect of heparin | Pyrococcus abyssi |
Cloned (Comment) | Organism |
---|---|
cloned into two expression vectors. The resulting 6His-tagged proteins are overexpressed in Escherichia coli XL-10-Gold or BL-21 (DE3) | Pyrococcus abyssi |
General Stability | Organism |
---|---|
complexes of the enzyme variants with either double- and single-stranded DNA fragments are less stable than the native DNA ligase | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | P0CL74 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus abyssi |
Synonyms | Comment | Organism |
---|---|---|
PabDBD | - |
Pyrococcus abyssi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
15 min, inactivated | Pyrococcus abyssi |