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Literature summary for 6.5.1.1 extracted from

  • Oscorbin, I.P.; Boyarskikh, U.A.; Zakabunin, A.I.; Khrapov, E.A.; Filipenko, M.L.
    DNA-binding domain of DNA ligase from the thermophilic archaeon Pyrococcus abyssi: improving long-range PCR and neutralization of heparin's inhibitory effect (2015), Appl. Biochem. Biotechnol., 176, 1859-1869.
    View publication on PubMed

Application

Application Comment Organism
molecular biology inclusion of the C-terminally 6His-tagged PabDBD in the reaction mixture during long-range polymerase chain reaction (PCR) increases the efficacy of amplification and eliminates the inhibitory effect of heparin Pyrococcus abyssi

Cloned(Commentary)

Cloned (Comment) Organism
cloned into two expression vectors. The resulting 6His-tagged proteins are overexpressed in Escherichia coli XL-10-Gold or BL-21 (DE3) Pyrococcus abyssi

General Stability

General Stability Organism
complexes of the enzyme variants with either double- and single-stranded DNA fragments are less stable than the native DNA ligase Pyrococcus abyssi

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi P0CL74
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus abyssi

Synonyms

Synonyms Comment Organism
PabDBD
-
Pyrococcus abyssi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
15 min, inactivated Pyrococcus abyssi