Literature summary for 6.5.1.1 extracted from

Samai, P.; Shuman, S.
Kinetic analysis of DNA strand joining by Chlorella virus DNA ligase and the role of nucleotidyltransferase motif VI in ligase adenylylation (2012), J. Biol. Chem., 287, 28609-28618.

Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	Paramecium bursaria chlorella virus

Protein Variants

Protein Variants	Comment	Organism
CDELTA1	mutant with deleted C-terminal five amino acids: mutant shows 34% the specific activity of wild-type. Preformed Lig-AMP in vivo: 71% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 8% of total enzyme (wild-type: 21%)	Paramecium bursaria chlorella virus
CDELTA2	mutant with deleted C-terminal five amino acids: mutant shows 6% the specific activity of wild-type. Preformed Lig-AMP in vivo: 80% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 1.1% of total enzyme (wild-type: 21%)	Paramecium bursaria chlorella virus
CDELTA3	mutant with deleted C-terminal five amino acids: mutant shows 2% the specific activity of wild-type. Preformed Lig-AMP in vivo: 67% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 1.3% of total enzyme (wild-type: 21%)	Paramecium bursaria chlorella virus
CDELTA4	mutant with deleted C-terminal five amino acids: mutant shows 1% the specific activity of wild-type. Preformed Lig-AMP in vivo: 18% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 0.8% of total enzyme (wild-type: 21%)	Paramecium bursaria chlorella virus
CDELTA5	mutant with deleted C-terminal five amino acids: mutant shows 1% the specific activity of wild-type and is poorly responsive to ATP. Preformed Lig-AMP in vivo: 7% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 0.5% of total enzyme (wild-type: 21%)	Paramecium bursaria chlorella virus
D297A	preformed Lig-AMP in vivo: 76% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 2.9% of total enzyme (wild-type: 21%)	Paramecium bursaria chlorella virus
D297E	preformed Lig-AMP in vivo: 74% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 1.8% of total enzyme (wild-type: 21%)	Paramecium bursaria chlorella virus
D297N	preformed Lig-AMP in vivo: 60% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 2.8% of total enzyme (wild-type: 21%)	Paramecium bursaria chlorella virus
R293A	preformed Lig-AMP in vivo: 52% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 1.9% of total enzyme (wild-type: 21%)	Paramecium bursaria chlorella virus
R293K	preformed Lig-AMP in vivo: 70% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 3.4% of total enzyme (wild-type: 21%)	Paramecium bursaria chlorella virus
R293Q	preformed Lig-AMP in vivo: 48% of total enzyme (wild-type: 70%), preformed Lig-AMP in vitro: 3.8% of total enzyme (wild-type: 21%)	Paramecium bursaria chlorella virus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.1	1	ATP	pH 7.5, 22°C, wild-type, Vmax: 1.1/sec	Paramecium bursaria chlorella virus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	10 mM, standard reaction condition	Paramecium bursaria chlorella virus

Organism

Organism	UniProt	Comment	Textmining
Paramecium bursaria chlorella virus	O41026	-	-

Purification (Commentary)

Purification (Comment)	Organism
using Ni-NTA chromatography	Paramecium bursaria chlorella virus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	-	Paramecium bursaria chlorella virus	AMP + diphosphate + (deoxyribonucleotide)n+m	-	?

Synonyms

Synonyms	Comment	Organism
ChVLig	-	Paramecium bursaria chlorella virus
DNA ligase	-	Paramecium bursaria chlorella virus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at	Paramecium bursaria chlorella virus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Paramecium bursaria chlorella virus

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Release 2023.1 (January 2023)