Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes in Escherichia coli | Pyrococcus furiosus |
Crystallization (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Protein Variants | Comment | Organism |
---|---|---|
D540A | mutant exhibits notably enhanced nick-joining activity compared with that of the wild type enzyme, the mutant enzyme exhibits activity about twice as high as that of the wild type within 10 min | Pyrococcus furiosus |
D540A/Q547A/K554A/K558A | the mutant enzyme exhibits activity about twice as high as that of the wild type within 10 min. The D540A ligation is almost the same as that of the D540A/Q547A/K554A/K558A mutant enzyme, thus implying that a single substitution for Asp540 might exert a more dominant effect than the substitutions of the other three polar and ionic residues at the C terminus | Pyrococcus furiosus |
D540K | the mutant exhibits notably enhanced nick-joining activity compared with that of the wild type enzyme | Pyrococcus furiosus |
D540R | the mutant exhibits notably enhanced nick-joining activity compared with that of the wild type enzyme | Pyrococcus furiosus |
D540S | the mutant exhibits notably enhanced nick-joining activity compared with that of the wild type enzyme | Pyrococcus furiosus |
Q547A/K554A/K558A | nick ligation activity of the mutant is slightly higher than that of the wild type enzyme | Pyrococcus furiosus |
R544A | mutant R544A displays a notable reduction in nick-joining activity (less than 45% of the input substrate ligated) in comparison with that of mutant R544A/Q547A/K554A/K558A | Pyrococcus furiosus |
R544A/Q547A/K554A/K558A | mutant enzyme exhibits low activity. Mutant R544A displays a notable reduction in nick-joining activity (less than 45% of the input substrate ligated) in comparison with that of mutant R544A/Q547A/K554A/K558A | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Thermococcus sp. | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
DNA ligase | - |
Thermococcus sp. |
PfuLig | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Pyrococcus furiosus |