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Literature summary for 6.5.1.1 extracted from

  • Samai, P.; Shuman, S.
    Structure-function analysis of the OB and latch domains of Chlorella virus DNA ligase (2011), J. Biol. Chem., 286, 22642-22652.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Chlorella virus

Protein Variants

Protein Variants Comment Organism
F190A the mutant has 31% of wild type nick sealing activity Chlorella virus
F215A the mutant has 70% of wild type nick sealing activity Chlorella virus
F215L the mutant has 75% of wild type nick sealing activity Chlorella virus
F276A/M278A the mutant has 42% of wild type nick sealing activity Chlorella virus
F286A the mutant has 4% of wild type nick sealing activity Chlorella virus
F286L the mutant has 61% of wild type nick sealing activity Chlorella virus
K274A the mutant has 34% of wild type nick sealing activity Chlorella virus
K274Q the mutant has 72% of wild type nick sealing activity Chlorella virus
K274R the mutant has 67% of wild type nick sealing activity Chlorella virus
K281A/C283A the mutant has 73% of wild type nick sealing activity Chlorella virus
N214A the mutant has 51% of wild type nick sealing activity Chlorella virus
N214D the mutant has 94% of wild type nick sealing activity Chlorella virus
N214L the mutant has 113% of wild type nick sealing activity Chlorella virus
N214Q the mutant has 98% of wild type nick sealing activity Chlorella virus
R285A the mutant has 7% of wild type nick sealing activity Chlorella virus
R285K the mutant has 6% of wild type nick sealing activity Chlorella virus
R285Q the mutant has 8% of wild type nick sealing activity Chlorella virus
S218A/R220A the mutant has 49% of wild type nick sealing activity Chlorella virus
S221A/T222A/H223A the mutant has 42% of wild type nick sealing activity Chlorella virus
S235A the mutant has 112% of wild type nick sealing activity Chlorella virus
S235A/K281A/C283A the mutant has 49% of wild type nick sealing activity Chlorella virus
T249A the mutant has 92% of wild type nick sealing activity Chlorella virus
V288A the mutant has 58% of wild type nick sealing activity Chlorella virus
V288I the mutant has 91% of wild type nick sealing activity Chlorella virus
V288T the mutant has 74% of wild type nick sealing activity Chlorella virus
Y217A the mutant has 44% of wild type nick sealing activity Chlorella virus
Y217F the mutant has 112% of wild type nick sealing activity Chlorella virus
Y217L the mutant has 84% of wild type nick sealing activity Chlorella virus
Y217S the mutant has 85% of wild type nick sealing activity Chlorella virus

Inhibitors

Inhibitors Comment Organism Structure
phosphate wild type enzyme activity is reduced by 4%, 35%, 73%, and 91% at 100 mM, 150 mM, 200 mM, and 250 mM phosphate, respectively Chlorella virus

Organism

Organism UniProt Comment Textmining
Chlorella virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-agarose column chromatography and phosphocellulose column chromatography Chlorella virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Chlorella virus AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Synonyms

Synonyms Comment Organism
Lig
-
 Chlorella virus

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Chlorella virus