Literature summary for 6.5.1.1 extracted from

Cotner-Gohara, E.; Kim, I.K.; Hammel, M.; Tainer, J.A.; Tomkinson, A.E.; Ellenberger, T.
Human DNA ligase III recognizes DNA ends bydynamic switching between two DNA-bound states (2010), Biochemistry, 49, 6165-6176.

Search for more literature for 6.5.1.1

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
LigIII in complex with 22-mer nicked DNA, hanging drop vapor diffusion method, using 1.8 M ammonium sulfate and 0.1 M sodium acetate (pH 5.6)	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
K323E	the mutant has significantly reduced blunt end DNA ligation activity (87% decrease in the initial velocity of blunt end DNA ligation compared to wild type enzyme), but has very little effect on DNA nick joining activity, the mutation selectively blocks zinc finger function	Homo sapiens
K323E/E265K	the mutations do not restore efficient blunt end DNA joining activity	Homo sapiens
R327E	the mutant is nearly devoid of blunt end joining activity, mimicking a deletion of the zinc finger	Homo sapiens
R327E/D262R	the mutations do not restore efficient blunt end DNA joining activity	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	the enzyme contains a PARP-like zinc finger	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
DNA ligase III	-	Homo sapiens
LigIII	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
AMP	-	Homo sapiens

General Information

General Information	Comment	Organism
physiological function	besides repairing nuclear DNA, ligase III is the only mitochondrial DNA ligase in which it functions in DNA repair and replication	Homo sapiens

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Release 2023.1 (January 2023)