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Literature summary for 6.5.1.1 extracted from

  • Keppetipola, N.; Shuman, S.
    Characterization of a thermophilic ATP-dependent DNA ligase from the euryarchaeon Pyrococcus horikoshii (2005), J. Bacteriol., 187, 6902-6908.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
ADP inhibits catalysis of nick-sealing by the ligase-AMP intermediate Pyrococcus horikoshii
Ca2+ partially inhibitory, ligation activity in presence of Mg2+ Pyrococcus horikoshii
Cd2+ 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ Pyrococcus horikoshii
Co2+ 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ Pyrococcus horikoshii
CTP 2 mM, 18% inhibition of single-turnover ligation Pyrococcus horikoshii
Cu2+ partially inhibitory, ligation activity in presence of Mg2+ Pyrococcus horikoshii
GTP 2 mM, 38% inhibition of single-turnover ligation Pyrococcus horikoshii
Mn2+ 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ Pyrococcus horikoshii
Ni2+ 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ Pyrococcus horikoshii
UTP 2 mM, 7% inhibition of single-turnover ligation Pyrococcus horikoshii
Zn2+ 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ Pyrococcus horikoshii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii
Cd2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii
Co2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii
Cu2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii
Mg2+ 1 mM, supports autoadenylation reaction. Required for nick-joining activity at 2-5 mM Pyrococcus horikoshii
Mn2+ 1 mM, supports autoadenylation reaction. Supports nick-joining activity to a much lesser extent than Mg2+ Pyrococcus horikoshii
Ni2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii
Zn2+ 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus horikoshii

Subunits

Subunits Comment Organism
monomer
-
Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
ATP-dependent DNA ligase
-
Pyrococcus horikoshii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
ligation reaction Pyrococcus horikoshii
90
-
adenylyltransferase activity Pyrococcus horikoshii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 90 60°C: about 35% of maximal activity, 90°C: about 90% of maximal activity, ligation activity Pyrococcus horikoshii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
adenylyltransferase activity Pyrococcus horikoshii

pH Range

pH Minimum pH Maximum Comment Organism
5 8.5 pH 5.0: about 65% of maximal activity, pH 8.5: about 45% of maximal activity, adenylyltransferase activity Pyrococcus horikoshii