Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | inhibits catalysis of nick-sealing by the ligase-AMP intermediate | Pyrococcus horikoshii | |
Ca2+ | partially inhibitory, ligation activity in presence of Mg2+ | Pyrococcus horikoshii | |
Cd2+ | 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ | Pyrococcus horikoshii | |
Co2+ | 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ | Pyrococcus horikoshii | |
CTP | 2 mM, 18% inhibition of single-turnover ligation | Pyrococcus horikoshii | |
Cu2+ | partially inhibitory, ligation activity in presence of Mg2+ | Pyrococcus horikoshii | |
GTP | 2 mM, 38% inhibition of single-turnover ligation | Pyrococcus horikoshii | |
Mn2+ | 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ | Pyrococcus horikoshii | |
Ni2+ | 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ | Pyrococcus horikoshii | |
UTP | 2 mM, 7% inhibition of single-turnover ligation | Pyrococcus horikoshii | |
Zn2+ | 5 mM, abolishes ligation reaction in presence of 5 mM Mg2+ | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM | Pyrococcus horikoshii | |
Cd2+ | 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM | Pyrococcus horikoshii | |
Co2+ | 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM | Pyrococcus horikoshii | |
Cu2+ | 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM | Pyrococcus horikoshii | |
Mg2+ | 1 mM, supports autoadenylation reaction. Required for nick-joining activity at 2-5 mM | Pyrococcus horikoshii | |
Mn2+ | 1 mM, supports autoadenylation reaction. Supports nick-joining activity to a much lesser extent than Mg2+ | Pyrococcus horikoshii | |
Ni2+ | 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM | Pyrococcus horikoshii | |
Zn2+ | 1 mM, supports autoadenylation reaction. Does not support nick-joining activity at 2-5 mM | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent DNA ligase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
ligation reaction | Pyrococcus horikoshii |
90 | - |
adenylyltransferase activity | Pyrococcus horikoshii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 90 | 60°C: about 35% of maximal activity, 90°C: about 90% of maximal activity, ligation activity | Pyrococcus horikoshii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
adenylyltransferase activity | Pyrococcus horikoshii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8.5 | pH 5.0: about 65% of maximal activity, pH 8.5: about 45% of maximal activity, adenylyltransferase activity | Pyrococcus horikoshii |