Cloned (Comment) | Organism |
---|---|
expression of intein-DNA ligase I fusion protein in Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
ATP | 37°C, pH 7.5 | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
87000 | - |
x * 87000, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Arabidopsis thaliana | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant intein-DNA ligase I fusion protein, chitin beads, Affi Gel Blue | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Arabidopsis thaliana | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
r | |
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA ligase I is able to ligate nicks in oligo(dT)/poly(dA) and oligo(rA)/poly(dT) substrates but not in oligo(dT)/poly(rA) substrates, double-stranded DNA's with cohesive or blunt ends are also good substrates | Arabidopsis thaliana | AMP + diphosphate + (deoxyribonucleotide)m+n | ability of DNA ligase I to revert the ligation reaction by relaxing the supercoiled DNA | r |
Subunits | Comment | Organism |
---|---|---|
? | x * 87000, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
DNA ligase I | - |
Arabidopsis thaliana |