Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus shibatae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | 100 mM, 85% inhibition, not inhibited below 50 mM | Saccharolobus shibatae | |
NaCl | 100 mM, 85% inhibition, not inhibited below 50 mM | Saccharolobus shibatae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
ATP | 65°C, pH 6.5, recombinant DNA-ligase, in the presence of 1mM ATP and 5 mM Mn2+ | Saccharolobus shibatae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent cation required for activity, most effectiv cation together with Mg2+ for native DNA ligase, maximal activity at 5 mM | Saccharolobus shibatae | |
Mg2+ | divalent cation required for activity, most effectiv cation together with Ca2+ for native DNA ligase, maximal activity at 5 mM | Saccharolobus shibatae | |
Mn2+ | divalent cation required for activity, most effectiv cation for recombinant DNA ligase, 10times higher efficiency than with Mg2+, maximal activity at 2-5 mM | Saccharolobus shibatae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
gel filtration | Saccharolobus shibatae |
67647 | - |
1 * 67647, deduced from nucleotide sequence | Saccharolobus shibatae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Saccharolobus shibatae | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | Q9P9K9 | - |
- |
Purification (Comment) | Organism |
---|---|
purification of recombinant DNA ligase, partially purification of native DNA ligase | Saccharolobus shibatae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Saccharolobus shibatae | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | very low activity | Saccharolobus shibatae | dAMP + diphosphate + (deoxyribonucleotide)m+n | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 67647, deduced from nucleotide sequence | Saccharolobus shibatae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 80 | - |
Saccharolobus shibatae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 90 | - |
Saccharolobus shibatae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
15% loss of activity after 5 h | Saccharolobus shibatae |
85 | - |
29% loss of activity after 5 h | Saccharolobus shibatae |
90 | - |
50% loss of activity after approx. 10 min | Saccharolobus shibatae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Saccharolobus shibatae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus shibatae | deduced from nucleotide sequence | - |
5.86 |