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Literature summary for 6.5.1.1 extracted from

  • Elder, R.H.; Rossignol, J.M.
    DNA ligases from rat liver. Purification and partial characterization of two molecular forms (1990), Biochemistry, 29, 6009-6017.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information antibodies raised against the 130000 MW polypeptide of DNA ligase I specifically recognize this species in an immunoblot and inhibit only the activity of DNA ligase I Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
ATP DNA ligase II Rattus norvegicus
0.06
-
ATP DNA ligase I Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
x * 100000, DNA ligase II, SDS-PAGE Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
DNA ligase I and II
-

Purification (Commentary)

Purification (Comment) Organism
DNA ligase I and II Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase II can use oligo(dT)*poly(rA) as substrate, DNA ligase I not Rattus norvegicus AMP + diphosphate + (deoxyribonucleotide)n+m
-
?
ATPalphaS + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase II can use ATPalphaS much more efficiently than DNA ligase I Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
monomer x * 100000, DNA ligase II, SDS-PAGE Rattus norvegicus