Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | antibodies raised against the 130000 MW polypeptide of DNA ligase I specifically recognize this species in an immunoblot and inhibit only the activity of DNA ligase I | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
ATP | DNA ligase II | Rattus norvegicus | |
0.06 | - |
ATP | DNA ligase I | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
x * 100000, DNA ligase II, SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
DNA ligase I and II | - |
Purification (Comment) | Organism |
---|---|
DNA ligase I and II | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA ligase II can use oligo(dT)*poly(rA) as substrate, DNA ligase I not | Rattus norvegicus | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
ATPalphaS + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA ligase II can use ATPalphaS much more efficiently than DNA ligase I | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | x * 100000, DNA ligase II, SDS-PAGE | Rattus norvegicus |