Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K+ | 50 mM, 50% inhibition | Crithidia fasciculata | |
NEM | - |
Crithidia fasciculata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00034 | - |
ATP | - |
Crithidia fasciculata |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Crithidia fasciculata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration, glycerol density gradient sedimentation | Crithidia fasciculata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DNA ligase I | Crithidia fasciculata |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Crithidia fasciculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | joins oligo(rA) annealed to poly(dT) with 250fold lower efficiency than it joins oligo(dT) annealed to poly(dA), ligates blunt-ended DNA fragments in the presence of 15% polyethylene glycol 80000 | Crithidia fasciculata | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | x * 80000, SDS-PAGE | Crithidia fasciculata |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
50 | - |
K+ | - |
Crithidia fasciculata |