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Literature summary for 6.4.1.6 extracted from

  • Rosier, C.; Leys, N.; Henoumont, C.; Mergeay, M.; Wattiez, R.
    Purification and characterization of the acetone carboxylase of Cupriavidus metallidurans strain CH34 (2012), Appl. Environ. Microbiol., 78, 4516-4518.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.4.1.6

Metals/Ions

Metals/Ions Comment Organism Structure
K+ stimulate the acetone carboxylase activity Cupriavidus metallidurans
KHCO3 stimulate the acetone carboxylase activity Cupriavidus metallidurans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
 SDS-PAGE, alpha subunit Cupriavidus metallidurans
76000
-
 SDS-PAGE, alpha subunit Cupriavidus metallidurans
86000
-
 SDS-PAGE, alpha subunit Cupriavidus metallidurans
388000
-
 gel filtration Cupriavidus metallidurans

Organism

Organism UniProt Comment Textmining
Cupriavidus metallidurans
-
 strain CH34
-

Purification (Commentary)

Purification (Comment) Organism
using anion DEAE-Sepharose chromatography followed by Sephacryl S300 molecular filtration Cupriavidus metallidurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-butanone + CO2 + ATP + H2O
-
 Cupriavidus metallidurans 2-methyl-3-oxobutanoate + AMP + phosphate
-
 ?
acetone + CO2 + ATP + H2O
-
 Cupriavidus metallidurans acetoacetate + AMP + phosphate
-
 ?

Subunits

Subunits Comment Organism
hexamer heterohexameric complex alpha2beta2gamma2, 2 * 86000 + 2 * 76000 + 2 * 19000, SDS-PAGE Cupriavidus metallidurans

Synonyms

Synonyms Comment Organism
acetone carboxylase
-
 Cupriavidus metallidurans
ACX
-
 Cupriavidus metallidurans

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8
-
 Cupriavidus metallidurans